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Literature summary for 1.14.11.2 extracted from
- Prolyl hydroxylase of earthworms. Substrate specificity of an enzyme from the subcuticular epithelium (1977), J. Biol. Chem., 252, 7591-7597.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation | Rattus norvegicus | |
| bovine serum albumin | activation | Lumbricus terrestris | |
| dithiothreitol | activation | Rattus norvegicus |  |
| dithiothreitol | activation | Lumbricus terrestris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Rattus norvegicus | |
| Fe2+ | - |
Lumbricus terrestris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lumbricus terrestris | - |
earthworm | - |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and calcium phosphate gel fractionation | Lumbricus terrestris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cuticle | - |
Lumbricus terrestris | - |
| skin | new born | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
- |
Lumbricus terrestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Gly-Ala-Pro)n + 2-oxoglutarate + O2 | less than 10% relative activity with respect to (Gly-Pro-Ala)n | Lumbricus terrestris | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| (Gly-Ala-Pro)n + 2-oxoglutarate + O2 | active substrate | Rattus norvegicus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| (Gly-Pro-Ala)n + 2-oxoglutarate + O2 | poor substrate | Rattus norvegicus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| (Gly-Pro-Ala)n + 2-oxoglutarate + O2 | the best substrate | Lumbricus terrestris | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| (Pro-Gly-Pro)n + 2-oxoglutarate + O2 | active substrate | Rattus norvegicus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| (Pro-Gly-Pro)n + 2-oxoglutarate + O2 | active substrate | Lumbricus terrestris | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| additional information | the enzyme clearly prefers X position proline to Y position proline in the sequences of (Gly-Pro-Ala)n versus (Gly-Ala-Pro)n | Lumbricus terrestris | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Rattus norvegicus | |
| 2-oxoglutarate | - |
Lumbricus terrestris | |
| ascorbate | - |
Rattus norvegicus | |
| ascorbate | - |
Lumbricus terrestris | |
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Release 2023.1 (January 2023)
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