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Literature summary for 1.14.11.2 extracted from

  • Chrispeels, M.J.
    Prolyl hydroxylase in plants (1984), Methods Enzymol., 107, 361-369.
No PubMed abstract available
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Catharanthus roseus
dithiothreitol activation Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
potassium phosphate
-
 Catharanthus roseus
Sodium acetate
-
 Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 O2
-
 Catharanthus roseus
0.23
-
 poly(L-proline)
-
 Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum 30% of the enzyme activity Lolium multiflorum 5783
-
Golgi apparatus 70% of the enzyme activity Lolium multiflorum 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Daucus carota
Fe2+
-
 Catharanthus roseus
Fe2+
-
 Lolium multiflorum

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-
Daucus carota
-
-
-
Lolium multiflorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using ammonium sulfate precipitation, column chromatography on DEAE-Sephadex A-50 and affinity chromatography on agarose-linked poly(L-proline) column Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2
-
 Catharanthus roseus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2
-
 ?
Gly-Pro8 + 2-oxoglutarate + O2 96% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Daucus carota ?
-
 ?
additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Catharanthus roseus ?
-
 ?
additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Lolium multiflorum ?
-
 ?
octa-L-proline + 2-oxoglutarate + O2 81.8% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2
-
 Lolium multiflorum poly(4-hydroxyproline) + succinate + CO2
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2 MW 2000, 6000 and 12000 Catharanthus roseus poly(4-hydroxyproline) + succinate + CO2
-
 ?
poly(L-proline) + 2-oxoglutarate + O2
-
 Catharanthus roseus poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
protocollagen + 2-oxoglutarate + O2
-
 Daucus carota 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
protocollagen + 2-oxoglutarate + O2
-
 Catharanthus roseus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
protocollagen + 2-oxoglutarate + O2
-
 Lolium multiflorum 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
tert-butyloxycarbonyl-Pro8 + 2-oxoglutarate + O2 91.4% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Catharanthus roseus
7.5
-
-
 Lolium multiflorum

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate Km: 0.09 mM Catharanthus roseus
ascorbate
-
 Daucus carota
ascorbate
-
 Catharanthus roseus
ascorbate
-
 Lolium multiflorum