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Literature summary for 1.14.11.2 extracted from

  • Majamaa, K.; Gunzler, V.; Hanauske-Abel, H.M.; Myllylä, R.; Kivirikko, K.I.
    Partial identity of the 2-oxoglutarate and ascorbate binding sites of prolyl 4-hydroxylase (1986), J. Biol. Chem., 261, 7819-7823.
    View publication on PubMed
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
(+)-mandelate
-
 Gallus gallus
(-)-mandelate
-
 Gallus gallus
1,2,3-trihydroxybenzene
-
 Gallus gallus
1,2-dihydroxybenzene competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1,3-dihydroxybenzene competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1,4-dihydroxybenzene
-
 Gallus gallus
2,3-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
2,4-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
2,5-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
2,6-dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
2-Hydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
3,4,5-Trihydroxybenzoate
-
 Gallus gallus
3,4-dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
3,4-dihydroxycinnamate competitive with respect to 2-oxoglutarate and ascorbate Gallus gallus
3,4-dihydroxymandelate competitive with respect to 2-oxoglutarate and ascorbate and noncompetitive with respect to Fe2+ Gallus gallus
3,4-Dihydroxyphenylacetate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
3,4-Dihydroxyphenylpropionate competitive with respect to 2-oxoglutarate and ascorbate Gallus gallus
3,5-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
3-hydroxybenzoate
-
 Gallus gallus
4-hydroxybenzoate competitive with respect to 2-oxoglutarate, and noncompetitive with respect to ascorbate Gallus gallus
Benzene 1,4-dicarboxylate competitive inhibition with respect to ascorbate Gallus gallus
L-galactono gamma-lactone
-
 Gallus gallus
phenylacetate
-
 Gallus gallus
Pyridine 2,4-dicarboxylate uncompetitive inhibition with respect to ascorbate Gallus gallus
Pyridine 2,5-dicarboxylate uncompetitive inhibition with respect to ascorbate Gallus gallus
pyridine 2-carboxylate uncompetitive inhibition with respect to ascorbate Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?
protocollagen + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
5,6-Isopropylideneascorbate can replace ascorbate Gallus gallus
ascorbate
-
 Gallus gallus
D-isoascorbate can replace ascorbate Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 3,4-dihydroxybenzoate
-
 Gallus gallus
0.009
-
 3,4-Dihydroxyphenylacetate
-
 Gallus gallus
0.009
-
 3,4-dihydroxymandelate
-
 Gallus gallus
0.013
-
 3,4-Dihydroxyphenylpropionate
-
 Gallus gallus
0.018
-
 3,4-dihydroxycinnamate
-
 Gallus gallus
0.025
-
 1,2-dihydroxybenzene
-
 Gallus gallus
0.03
-
 3,4,5-Trihydroxybenzoate
-
 Gallus gallus
0.03
-
 1,2,3-trihydroxybenzene
-
 Gallus gallus
0.07
-
 2,3-Dihydroxybenzoate
-
 Gallus gallus
0.53
-
 2,4-Dihydroxybenzoate
-
 Gallus gallus
1.1
-
 2,6-dihydroxybenzoate
-
 Gallus gallus
1.4
-
 2,5-Dihydroxybenzoate
-
 Gallus gallus
2.3
-
 2-Hydroxybenzoate
-
 Gallus gallus
3.2
-
 4-hydroxybenzoate
-
 Gallus gallus
9
-
 3,5-Dihydroxybenzoate
-
 Gallus gallus
10.2
-
 1,3-dihydroxybenzene
-
 Gallus gallus
14
-
 1,4-dihydroxybenzene
-
 Gallus gallus
20
-
 phenylacetate value above Gallus gallus
20
-
 3-hydroxybenzoate value above Gallus gallus
20
-
 (-)-mandelate value above Gallus gallus
20
-
 L-galactono gamma-lactone value above Gallus gallus
20
-
 (+)-mandelate value above Gallus gallus