Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Gallus gallus | |
catalase | activation | Gallus gallus | |
dithiothreitol | activation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoadipinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
2-oxobutyrate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
2-oxosuccinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+; i.e. oxaloacetate | Gallus gallus | |
2-oxovalerate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
3-oxoglutarate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
adipinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
benzene 1,2-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Benzene 1,3-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Benzene 1,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
benzoate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Glutarate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
levulinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
malonate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
pyridine 2,3-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Pyridine 2,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Pyridine 2,5-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
pyridine 2,6-dicarboxylate | competitive with respect to Fe2+ and noncompetitive with respect to 2-oxoglutarate | Gallus gallus | |
pyridine 2-carboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Pyridine 3,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Pyridine 3,5-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
pyridine 3-carboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
Pyridine 4-carboxylate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
pyruvate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus | |
succinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
carboxymethylated collagen L-proline | as cosubstrate | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
carboxymethylated collagen L-proline + 2-oxoglutarate + O2 | - |
Gallus gallus | carboxymethylated collagen-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Gallus gallus | ? | - |
? | |
additional information | characterization of the 2-oxoglutarate binding site of the enzyme, 2-oxosuccinate, 2-oxovalerate, 2-oxobutyrate, 3-oxoglutarate or pyridine 2,5-dicarboxylate do not replace 2-oxoglutarate as cosubstrates | Gallus gallus | ? | - |
? | |
protocollagen + 2-oxoglutarate + O2 | 2-oxoadipate can replace 2-oxoglutarate as cosubstrate | Gallus gallus | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
Pyridine-2,5-dicarboxylate | - |
Gallus gallus | |
0.002 | - |
Pyridine-2,4-dicarboxylate | - |
Gallus gallus | |
0.025 | - |
pyridine 2-carboxylate | - |
Gallus gallus | |
0.1 | - |
2-oxosuccinate | - |
Gallus gallus | |
0.21 | - |
pyridine 2,3-dicarboxylate | - |
Gallus gallus | |
0.4 | - |
succinate | - |
Gallus gallus | |
0.5 | - |
pyridine 3-carboxylate | - |
Gallus gallus | |
0.8 | - |
3-oxoglutarate | - |
Gallus gallus | |
0.9 | - |
2-oxoadipinate | - |
Gallus gallus | |
0.9 | - |
Pyridine 3,5-dicarboxylate | - |
Gallus gallus | |
0.9 | - |
Pyridine 4-carboxylate | - |
Gallus gallus | |
2.2 | - |
Pyridine 3,4-dicarboxylate | - |
Gallus gallus | |
3.3 | - |
Benzene 1,4-dicarboxylate | - |
Gallus gallus | |
4.2 | - |
Glutarate | - |
Gallus gallus | |
5.8 | - |
Benzene 1,3-dicarboxylate | - |
Gallus gallus | |
5.9 | - |
malonate | - |
Gallus gallus | |
7.6 | - |
2-oxobutyrate | - |
Gallus gallus | |
9.4 | - |
2-oxovalerate | - |
Gallus gallus | |
9.9 | - |
benzoate | - |
Gallus gallus | |
10 | - |
benzene 1,2-dicarboxylate | value above | Gallus gallus | |
15 | - |
pyruvate | - |
Gallus gallus | |
15 | - |
levulinate | value above | Gallus gallus | |
15 | - |
adipinate | value above | Gallus gallus |