Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Gallus gallus | |
catalase | activation | Gallus gallus | |
dithiothreitol | activation | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
tert-butyloxycarbonyl-Val-Pro-Gly-Val-OH | - |
Gallus gallus | |
22.2 | - |
tert-butyloxycarbonyl-Gly-Val-Pro-Gly-Val-OH | - |
Gallus gallus | |
24 | - |
tert-butyloxycarbonyl-Pro-Pro-Ala-Pro-OH | - |
Gallus gallus | |
32.2 | - |
tert-butyloxycarbonyl-Pro-Pro-Gln-Pro-OCH3 | - |
Gallus gallus | |
37.2 | - |
tert-butyloxycarbonyl-L-Pro-L-Pro-Gly | - |
Gallus gallus | |
37.4 | - |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro-Pro-OH | - |
Gallus gallus | |
38.9 | - |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro-OH | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography, ion-exchange chromatography and gel filtration | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | structural requirement for proline hydroxylation in collagen and related peptides: supersecondary structure, consisting of PP-II followed by a beta-turn, as the conformational determinant for proline hydroxylation in nascent collagen and related substrates of the enzyme, in elastin peptides, the beta-structure appears to substitute for the PP-II structure | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
proline containing peptide + 2-oxoglutarate + O2 | synthetic peptides | Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Gly-Val-Pro-Gly-Val-OH + 2-oxoglutarate + O2 | - |
Gallus gallus | tert-butyloxycarbonyl-Gly-Val-Hyp-Gly-Val-OH + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-L-Pro-L-Pro-Gly + 2-oxoglutarate + O2 | - |
Gallus gallus | tert-butyloxycarbonyl-L-Pro-L-Pro-Gly-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
tert-butyloxycarbonyl-Pro-Pro-Ala-Pro-OH + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Pro-Pro-Gln-Pro-OCH3 + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro-NHCH3 + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro-Pro-OH + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Val-Pro-Gly-Val-OH + 2-oxoglutarate + O2 | - |
Gallus gallus | tert-butyloxycarbonyl-Val-Hyp-Gly-Val-OH + succinate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
tert-butyloxycarbonyl-Pro-Pro-Gln-Pro-OCH3 | - |
Gallus gallus | |
0.11 | - |
tert-butyloxycarbonyl-Pro-Pro-Ala-Pro-OH | - |
Gallus gallus | |
0.2 | - |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro | - |
Gallus gallus | |
0.38 | - |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro-NHCH3 | - |
Gallus gallus | |
0.79 | - |
tert-butyloxycarbonyl-Pro-Pro-Gly-Pro-Pro-OH | - |
Gallus gallus | |
0.88 | - |
tert-butyloxycarbonyl-Val-Pro-Gly-Val-OH | - |
Gallus gallus | |
2.33 | - |
tert-butyloxycarbonyl-Gly-Val-Pro-Gly-Val-OH | - |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Gallus gallus |