Cloned (Comment) | Organism |
---|---|
expressed in HEK293T cells and in Escherichia coli | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide L-asparagine + 2-oxoglutarate + O2 | Homo sapiens | hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300 | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
HIF (L-Asn803) + 2-oxoglutarate + O2 | accepts HIF-1alpha and HIF-2-alpha as substrate, HIF mutant V802A exhibits 4fold lower substrate activity than native protein, no activity with N803A mutant | Homo sapiens | HIF (3-hydroxy-L-Asn803) + succinate + CO2 | - |
? | |
peptide L-asparagine + 2-oxoglutarate + O2 | - |
Homo sapiens | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? | |
peptide L-asparagine + 2-oxoglutarate + O2 | hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300 | Homo sapiens | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor inhibiting hypoxia-inducible transcription factor (HIF) | - |
Homo sapiens |
FIH | - |
Homo sapiens |