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Literature summary for 1.14.11.15 extracted from

  • Lester, D.R.; Phillips, A.; Hedden, P.; Andersson, I.
    Purification and kinetic studies of recombinant gibberellin dioxygenases (2005), BMC Plant Biol., 5, 19.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00082
-
 gibberellin a20 in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39100
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C971
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilised metal affinity chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0216
-
 wild type enzyme from soluble cell fraction, in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase Arabidopsis thaliana
0.259
-
 wild type enzyme after 12fold purification, in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin a20 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana gibberellin a1 + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
GA 3-oxidase
-
 Arabidopsis thaliana
GA3OX4
-
 Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.3
-
 80% activity in phosphate buffer at pH 6.3 Arabidopsis thaliana