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Literature summary for 1.14.11.11 extracted from
- Biochemical and structural characterization of recombinant hyoscyamine 6beta-hydroxylase from Datura metel L (2010), Plant Physiol. Biochem., 48, 966-970.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DmH6H gene, expression of the His-tagged enzyme in Escherichia coli | Datura metel |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
2-oxoglutarate | pH 7.8, 30°C, recombinant enzyme | Datura metel |  |
| 0.05 | - |
L-hyoscyamine | pH 7.8, 30°C, recombinant enzyme | Datura metel | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Datura metel | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
x * 39000, recombinant His-tagged enzyme, SDS-PAGE | Datura metel |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-hyoscyamine + 2-oxoglutarate + O2 | Datura metel | - |
(6S)-hydroxyhyoscyamine + succinate + CO2 | H6H produces 6beta-hydroxyhyoscyamine and its derivative, scopolamine, the DmrH6H epoxidase activity is low compared to the hydroxylase activity | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Datura metel | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Datura metel |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-hyoscyamine + 2-oxoglutarate + O2 | - |
Datura metel | (6S)-hydroxyhyoscyamine + succinate + CO2 | H6H produces 6beta-hydroxyhyoscyamine and its derivative, scopolamine, the DmrH6H epoxidase activity is low compared to the hydroxylase activity | ? | |
| L-hyoscyamine + 2-oxoglutarate + O2 | the binding of the substrates, hyoscyamine and 2-oxoglutarate, to the enzyme induces significant conformational changes | Datura metel | (6S)-hydroxyhyoscyamine + succinate + CO2 | H6H produces 6beta-hydroxyhyoscyamine and its derivative, scopolamine, the DmrH6H epoxidase activity is low compared to the hydroxylase activity | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, recombinant His-tagged enzyme, SDS-PAGE | Datura metel |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| H6H | - |
Datura metel |
| hyoscyamine 6beta-hydroxylase | - |
Datura metel |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Datura metel |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Datura metel |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | hyoscyamine 6b-hydroxylase, H6H, is an important enzyme in the biosynthesis of tropane alkaloids. It catalyzes the hydroxylation of hyoscyamine to give 6beta-hydroxyhyoscyamine and its epoxidation in the biosynthetic pathway leading to scopolamine. The DmrH6H epoxidase activity Is low compared to the hydroxylase activity | Datura metel |
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