Cloned (Comment) | Organism |
---|---|
the gene encoding hyosyamine 6-hydroxylase is placed under the regulation of the CaMV 35S promoter and introduced into the genome of a scopolamine-rich Duboisia hybrid by a binary vector system using Agrobacterium rhizogenes strain LBA9402. The scopolamine levels in the resulting engineered hairy root lines increases up to three times compared to wild-type hairy roots, but there is no clear increase in the engineered regenerated plants | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | - |
scopolamine + succinate + CO2 | - |
? | |
L-hyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | - |
6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
L-hyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? |