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Literature summary for 1.14.11.10 extracted from
- Identification of two alpha-ketoglutarate-dependent dioxygenases in extracts of Rhodotorula glutinis catalyzing deoxyuridine hydroxylation (1985), J. Biol. Chem., 260, 9972-9975.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rhodotorula glutinis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | requirement | Rhodotorula glutinis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyuridine + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
uracil + deoxyribonolactone + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula glutinis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rhodotorula glutinis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least a month in 0.02 M sodium phosphate buffer, pH 7.5, with 0.1 mM EDTA | Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-2'-fluorouridine + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | uracil + 2-fluororibonolactone + succinate + CO2 | - |
? | |
| 2'-deoxyuridine + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | uracil + deoxyribonolactone + succinate + CO2 | - |
? | |
| uridine + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | ribonolactone + uracil + succinate + CO2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rhodotorula glutinis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rhodotorula glutinis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | requirement | Rhodotorula glutinis | |
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Release 2023.1 (January 2023)
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