Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Pseudomonas fluorescens | |
indole-3-methanol | inhibits oxidation of skatole | Pseudomonas fluorescens | |
KCN | - |
Pseudomonas fluorescens | |
skatole | inhibits oxidation of indole-3-methanol | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
indole-3-methanol | - |
Pseudomonas fluorescens | |
0.67 | - |
ferricyanide | - |
Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | dehydrogenase component: 0.67 atoms of iron per mol of component | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
72000 | - |
1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
ATCC 29574 | - |
Purification (Comment) | Organism |
---|---|
dehydrogenase component and oxidase component | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.2 | - |
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-indolemethanol + ferricyanide | - |
Pseudomonas fluorescens | ? | - |
r | |
3-indolemethanol + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
additional information | dehydrogenase component abstracts electrons from the substrate and transfers them to oxidation-reduction dyes (e.g. potassium ferricyanide, 2,6-dichlorophenolindophenol) but not to molecular oxygen, the oxidase component transfers electrons from the former component to oxygen | Pseudomonas fluorescens | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
dimer | dehydrogenase component abstracts electrons from the substrate and transfers them to oxidation-reduction dyes e.g. potassium ferricyanide, 2,6-dichlorophenolindophenol, but not to molecular oxygen, the oxidase component transfers electrons from the former component to oxygen | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | hemoprotein | Pseudomonas fluorescens | |
heme | dehydrogenase component: 0.73 mol protoheme IX per mol of component | Pseudomonas fluorescens |