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Literature summary for 1.13.99.1 extracted from
- Purification, characterization and functional cloning of inositol oxygenase from Cryptococcus (2003), Yeast, 20, 1317-1329.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Sporopachydermia lactativora |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Sporopachydermia lactativora |  |
| p-chloromercuribenzoate | - |
Sporopachydermia lactativora | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
O2 | 30°C, pH 6.5 | Sporopachydermia lactativora | |
| 5 | - |
myo-inositol | 30°C, pH 6.5 | Sporopachydermia lactativora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Sporopachydermia lactativora |
| 37000 | - |
1 * 37000, SDS-PAGE | Sporopachydermia lactativora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | Sporopachydermia lactativora | - |
D-glucuronic acid + H2O | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sporopachydermia lactativora | Q70GL3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-column, butyl-Sepharose, Superdex 75, Mono Q | Sporopachydermia lactativora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | - |
Sporopachydermia lactativora | D-glucuronic acid + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, SDS-PAGE | Sporopachydermia lactativora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| InOx | - |
Sporopachydermia lactativora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Sporopachydermia lactativora |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
60 | approx. 30% of maximal activity at 0°C and 50°C, respectively | Sporopachydermia lactativora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Sporopachydermia lactativora |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | almost no activity at pH 4.5, approx. 10% of maximal ativity at pH 10.0, approx. 70% of maximal activity at pH 8.0, i.e. a second optima | Sporopachydermia lactativora |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
p-chloromercuribenzoate | - |
Sporopachydermia lactativora | |
| 0.02 | - |
1,10-phenanthroline | - |
Sporopachydermia lactativora | |
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