Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Photinus pyralis |
General Stability | Organism |
---|---|
sorbitol, proline and sucrose with strong stabilizing effect on firefly luciferase activity at 35°C | Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose, yield of 95% | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luciferin + O2 + ATP | assay at pH 7.8 | Photinus pyralis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Photinus pyralis |
30 | - |
in presence of sucrose, proline or sorbitol | Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at 40°C | Photinus pyralis |
35 | - |
about 70% loss of activity within 10 min, no residual activity after 60 min, in presence of osmolytes higher residual activity after 60 min: 90% with 1.5 M sucrose, 50% with 1.5 M sorbitol, 35% with 1.5 M proline | Photinus pyralis |