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Literature summary for 1.13.12.7 extracted from
- Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions (2010), Cell. Mol. Life Sci., 67, 387-404.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | essential for luminescence reaction | Luciola cruciata |  |
| ATP | essential for luminescence reaction | Pyrophorus plagiophthalamus | |
| ATP | essential for luminescence reaction, initiates luminescence reaction | Photinus pyralis | |
| CoA | prevents rapid decay of light emission | Photinus pyralis | |
| diphosphate | stimulates light production under some conditions | Photinus pyralis | |
| molecular oxygen | essential for luminescence reaction | Photinus pyralis | |
| molecular oxygen | essential for luminescence reaction | Luciola cruciata | |
| molecular oxygen | essential for luminescence reaction | Pyrophorus plagiophthalamus | |
| Triphosphate | stimulates light production under some conditions | Photinus pyralis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Photinus pyralis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Photinus pyralis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | chimeric protein with fatty acyl-CoA synthetase, EC 6.2.1.3, 4% of luminescence activity from wild-tpe luciferase activity | Photinus pyralis |
| S286N | red bioluminescence | Luciola cruciata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-phenylbenzothiazole | - |
Photinus pyralis | |
| 5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | competitive inhibitor | Photinus pyralis | |
| AMP | - |
Photinus pyralis | |
| arachidonic acid | concentration above 10 microM | Photinus pyralis | |
| ATP | - |
Photinus pyralis | |
| bromoform | - |
Photinus pyralis | |
| dehydroluciferin | - |
Luciola cruciata | |
| dehydroluciferin | - |
Photinus pyralis | |
| dehydroluciferyl adenylate | - |
Luciola cruciata | |
| dehydroluciferyl adenylate | - |
Photinus pyralis | |
| diphosphate | depending on conditions | Photinus pyralis | |
| Halothane | non-competitive inhibitor | Photinus pyralis | |
| L-luciferin | - |
Luciola cruciata | |
| L-luciferin | - |
Photinus pyralis | |
| linoleic acid | concentration above 10 microM | Photinus pyralis | |
| lipoic acid | - |
Photinus pyralis | |
| myristic acid | - |
Photinus pyralis | |
| N-pyridin-2-ylbenzamide | - |
Photinus pyralis | |
| oleic acid | concentration above 10 microM | Photinus pyralis | |
| oxyluciferin | - |
Luciola cruciata | |
| oxyluciferin | - |
Photinus pyralis | |
| pifithrin-alpha | 20 microM, above 95% inhibition | Photinus pyralis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
D-luciferin | native luciferase | Photinus pyralis | |
| 0.0024 | - |
D-luciferin | native luciferase | Luciola cruciata | |
| 0.0047 | - |
D-luciferin | recombinant luciferase | Photinus pyralis | |
| 0.0047 | - |
D-luciferin | recombinant luciferase | Luciola cruciata | |
| 0.00741 | - |
lauric acid | - |
Photinus pyralis | |
| 0.0136 | - |
linoleic acid | - |
Photinus pyralis | |
| 0.015 | - |
D-luciferin | - |
Photinus pyralis | |
| 0.0163 | - |
lauric acid | - |
Luciola cruciata | |
| 0.16 | - |
ATP | - |
Photinus pyralis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | essential | Photinus pyralis | |
| Mg2+ | essential | Luciola cruciata | |
| Mg2+ | essential | Pyrophorus plagiophthalamus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquatica lateralis | Q01158 | - |
- |
| Cratomorphus distinctus | Q5USC8 | - |
- |
| Hotaria unmunsana | Q8T6U3 | - |
- |
| Lampyris noctiluca | Q27688 | - |
- |
| Lampyris turkestanicus | Q5UFR2 | - |
- |
| Lampyroidea maculata | Q1WLP6 | - |
- |
| Luciola cruciata | P13129 | - |
- |
| Luciola mingrelica | Q26304 | - |
- |
| Luciola parvula | Q25118 | - |
- |
| Photinus pyralis | P08659 | - |
- |
| Photuris pensylvanica | Q27757 | - |
- |
| Phrixothrix hirtus | Q9U4U7 | - |
- |
| Phrixothrix vivianii | Q9U4U8 | - |
- |
| Pyrearinus termitilluminans | AF116843 | - |
- |
| Pyrocoelia miyako | Q26076 | - |
- |
| Pyrocoelia rufa | Q9GPF9 | - |
- |
| Pyrophorus mellifluus | Q717B6 | - |
- |
| Pyrophorus plagiophthalamus | Q718F0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC | Luciola cruciata |
| ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC | Aquatica lateralis |
| gel filtration, affinity chromatography | Photinus pyralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
lower luminescence activity when another substrate than D-luciferin is used: only 10% luminescence activity with 6'-amino-D-luciferin, only 5% luminescence activity with 4'-methyl-D-luciferin, only 7% luminescence activity with D-quinolylluciferin, only 1.5% luminescence activity with D-naphthylluciferin, only 16% luminescence activity with D-5,5-dimethylluciferyl-adenylate | Photinus pyralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4'-methyl-D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
| 5-methylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
| 6'-amino-D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
| D-5,5-dimethylluciferyl-adenylate + O2 | yellow-green color of luminescence | Photinus pyralis | ? | - |
? | |
| D-5,5-dimethylluciferyl-adenylate + O2 + ATP | red color of luminescence | Pyrophorus plagiophthalamus | ? | - |
? | |
| D-luciferin + ATP + O2 | - |
Luciola cruciata | luciferyl-adenylate + diphosphate | - |
r | |
| D-luciferin + ATP + O2 | - |
Photinus pyralis | luciferyl-adenylate + diphosphate + H2O + hv | - |
r | |
| D-naphthylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
| D-quinolylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
| dehydroluciferin + ATP | - |
Photinus pyralis | dehydroluciferyl-AMP + diphosphate | - |
r | |
| dehydroluciferin + ATP | - |
Luciola cruciata | dehydroluciferyl-AMP + diphosphate | - |
r | |
| lauric acid + ATP + CoA | - |
Photinus pyralis | lauroyl-CoA + AMP + diphosphate | - |
? | |
| lauric acid + ATP + CoA | - |
Luciola cruciata | lauroyl-CoA + AMP + diphosphate | - |
? | |
| linoleic acid + ATP + CoA | linoleic acid concentration above 10 microM has an inhibitory effect | Photinus pyralis | linoleoyl-CoA + AMP + diphosphate | - |
? | |
| luciferyl-adenylate + O2 | - |
Photinus pyralis | oxyluciferin + AMP + CO2 + hv | - |
ir | |
| luciferyl-adenylate + O2 | - |
Luciola cruciata | oxyluciferin + AMP + CO2 + hv | - |
ir | |
| R-COOH + ATP | - |
Photinus pyralis | R-CO-AMP + diphosphate | - |
r | |
| R-COOH + ATP | - |
Luciola cruciata | R-CO-AMP + diphosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| firefly luciferase | - |
Photinus pyralis |
| firefly luciferase | - |
Luciola cruciata |
| luciferase | - |
Cratomorphus distinctus |
| luciferase | - |
Phrixothrix vivianii |
| luciferase | - |
Phrixothrix hirtus |
| luciferase | - |
Lampyris turkestanicus |
| luciferase | - |
Luciola mingrelica |
| luciferase | - |
Pyrophorus plagiophthalamus |
| luciferase | - |
Aquatica lateralis |
| luciferase | - |
Photuris pensylvanica |
| luciferase | - |
Pyrocoelia miyako |
| luciferase | - |
Pyrocoelia rufa |
| luciferase | - |
Luciola parvula |
| luciferase | - |
Hotaria unmunsana |
| luciferase | - |
Lampyris noctiluca |
| luciferase | - |
Lampyroidea maculata |
| luciferase | - |
Pyrophorus mellifluus |
| luciferase | - |
Pyrearinus termitilluminans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
red emitted light | Photinus pyralis |
| 7.6 | - |
yellow-green emitted light | Photinus pyralis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Photinus pyralis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Photinus pyralis | |
| CoA | - |
Photinus pyralis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
dehydroluciferyl adenylate | value between 0.0000025 - 0.00125 mM | Luciola cruciata | |
| additional information | - |
5'-O-[hydroxy({[(2Z)-2-(6-oxo-1,3-benzothiazol-2(6H)-ylidene)-2,3-dihydro-1,3-thiazol-4-yl]carbonyl}oxy)phosphoryl]adenosine | value between 0.0000025-0.00125 mM | Photinus pyralis | |
| additional information | - |
oxyluciferin | value between 0.0005-0.002 mM | Photinus pyralis | |
| additional information | - |
oxyluciferin | value between 0.0005-0.002 mM | Luciola cruciata | |
| additional information | - |
L-luciferin | value between 0.003-0.004 mM | Luciola cruciata | |
| 0.000026 | - |
lipoic acid | - |
Photinus pyralis | |
| 0.000034 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for D-luciferin | Photinus pyralis | |
| 0.000041 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for MgATP2- | Photinus pyralis | |
| 0.00034 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for luciferyl adenylate | Photinus pyralis | |
| 0.00053 | - |
myristic acid | - |
Photinus pyralis | |
| 0.003 | 0.004 | L-luciferin | - |
Photinus pyralis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00005 | - |
- |
Photinus pyralis | lipoic acid | |
| 0.000069 | - |
- |
Photinus pyralis | N-pyridin-2-ylbenzamide | |
| 0.00068 | - |
- |
Photinus pyralis | myristic acid | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme | Photinus pyralis |
| physiological function | fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme | Luciola cruciata |
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Release 2023.1 (January 2023)
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