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Literature summary for 1.13.12.7 extracted from

  • Yousefi-Nejad, M.; Hosseinkhani, S.; Khajeh, K.; Ranjbar, B.
    Expression, purification and immobilization of firefly luciferase on alkyl-substituted Sepharose 4B (2007), Enzyme Microb. Technol., 40, 740-746.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Photinus pyralis

General Stability

General Stability Organism
immobilization on hexyl-Sepharose reduces the specific activity to 35%, immobilization on octyl-Sepharose reduces the specific activity to 26% Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
ATP free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol Photinus pyralis
0.008
-
Luciferin free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis Q27758
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Photinus pyralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme from crude extract shows a specific activity of 6100000 relative light units per mg, purified enzyme shows a specific activity of 104000000 relative light units per mg Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-anilino-1-naphthalene-sulfonate + ?
-
Photinus pyralis ?
-
?
luciferin + O2 + ATP
-
Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Photinus pyralis
Luc
-
Photinus pyralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Photinus pyralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
immobilized enzyme forms are stable for 1 min at 40°C and then lose their activities Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Photinus pyralis