Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Photinus pyralis |
General Stability | Organism |
---|---|
immobilization on hexyl-Sepharose reduces the specific activity to 35%, immobilization on octyl-Sepharose reduces the specific activity to 26% | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
ATP | free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol | Photinus pyralis | |
0.008 | - |
Luciferin | free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | Q27758 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Photinus pyralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme from crude extract shows a specific activity of 6100000 relative light units per mg, purified enzyme shows a specific activity of 104000000 relative light units per mg | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-anilino-1-naphthalene-sulfonate + ? | - |
Photinus pyralis | ? | - |
? | |
luciferin + O2 + ATP | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
Luc | - |
Photinus pyralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
immobilized enzyme forms are stable for 1 min at 40°C and then lose their activities | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Photinus pyralis |