Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a chimeric protein with the N-terminal domain of firefly luciferase and the C-terminal domain of the firefly luciferase homolog from Drosophila melanogaster CG6178 shows approximately 4% of the activity of wild type firefly luciferase | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65500 | - |
calculated from amino acid sequence | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | P08659 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelate affinity chromatography | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + O2 + ATP | - |
Photinus pyralis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
fatty acid + ATP + CoA | - |
Photinus pyralis | fatty acyl-CoA + AMP | - |
? | |
luciferyl-O-adenosine monophosphate + CoA | - |
Photinus pyralis | luciferyl-CoA + AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
Photinus pyralis luciferase | - |
Photinus pyralis |
PpLase | - |
Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Photinus pyralis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Photinus pyralis | calculated with ExPASy Compute pI/MW tool | - |
6.2 |