Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Luciferin | preparations supplemented with extra luciferin provide maximum light emission | Photinus pyralis |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Photinus pyralis |
EDTA, 2-mercaptoethanol, dithiothreitol and bovine serum albumin used as stabilizers | Photinus pyralis |
more stable in phosphate buffer than in Tricine buffer | Photinus pyralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenate | reversible inhibition | Photinus pyralis | |
bovine serum albumin | inhibition when present in large excess | Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Photinus luciferin + O2 + ATP | - |
Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
higher stability in frozen state than in refrigerated | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
phosphate buffer | Photinus pyralis |