Literature summary for 1.13.12.6 extracted from

Wu, C.; Kawasaki, K.; Ogawa, Y.; Yoshida, Y.; Ohgiya, S.; Ohmiya, Y.
Preparation of biotinylated Cypridina luciferase and its use in bioluminescent enzyme immunoassay (2007), Anal. Chem., 79, 1634-1638.

Search for more literature for 1.13.12.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae	Cypridina noctiluca

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	biotin-CLuc, SDS-PAGE	Cypridina noctiluca

Organism

Organism	UniProt	Comment	Textmining
Cypridina noctiluca	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni affinity column chromatography, SA beads column chromatography, monomeric avidin column chromatography, and TSK G300SW gel filtration	Cypridina noctiluca

Storage Stability

Storage Stability	Organism
4°C, PBS buffer containing 0.1 M potassium phosphate (pH 7.2) and 0.15 M NaCl, 2 months, without significant loss of activity	Cypridina noctiluca

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Cypridina luciferin + O2	-	Cypridina noctiluca	oxidized Cypridina luciferin + CO2 + hv	-	?

Synonyms

Synonyms	Comment	Organism
CLuc	-	Cypridina noctiluca
Cypridina luciferase	-	Cypridina noctiluca

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	The biotinylated luciferase is stable when stored at 4°C.	Cypridina noctiluca

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Release 2023.1 (January 2023)