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Literature summary for 1.13.12.5 extracted from
- Recombinant Gaussia luciferase: Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization (2002), Anal. Chem., 74, 4378-4385.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | complexation of GLuc with streptavidin after biotinylation of GLuc | Gaussia princeps |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | fusion of GLuc to a biotin acceptor peptide, attachment of a single biotin to the fusion protein mediated by the biotin protein ligase, EC 6.3.4.15 | Gaussia princeps |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| coelenteramide | - |
Gaussia princeps |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18800 | - |
SDS-PAGE | Gaussia princeps |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gaussia princeps | Q9BLZ2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography | Gaussia princeps |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coelenterazine + O2 | - |
Gaussia princeps | coelenteramide + CO2 + hnu | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gaussia luciferase | - |
Gaussia princeps |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Gaussia princeps |
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Release 2023.1 (January 2023)
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