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Literature summary for 1.13.12.5 extracted from

  • Deng, L.; Vysotski, E.S.; Markova, S.V.; Liu, Z.J.; Lee, J.; Rose, J.; Wang, B.C.
    All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin (2005), Protein Sci., 14, 663-675.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
microbatch method is used for crystallization. The crystals of Ca2+-loaded apoaequorin are grown from 0.02 M CaCl2, 30% v/v 2-methyl-2,4-pentanediol, and 0.1 M sodium acetate (pH 4.6) during less than 1 week of incubation at 4°C. The maximum size of crystals is 0.35 * 0.3 * 0.25 mm Aequorea victoria

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ all three Ca2+-binding loops of photoproteins bind calcium ions Aequorea victoria

Organism

Organism UniProt Comment Textmining
Aequorea victoria P07164 aequorin-1 precursor
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroperoxycoelenterazine + O2
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Aequorea victoria ?
-
?

Synonyms

Synonyms Comment Organism
aequorin-1
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Aequorea victoria