Application | Comment | Organism |
---|---|---|
molecular biology | when aequorin is microinjected into cleavage-stage zebrafish embryos, it is largely used up by about 24 hours. Thus, it is not possible to image Ca2+ signals from later stages of zebrafish development using this approach. Transient expression of apoaequorin (i.e., the protein component of aequorin) using aeq-mRNA in zebrafish embryos and then reconstitution of intact aequorin in vivo by loading the coelenterazine cofactor into the embryos separately provides a valuable tool for monitoring Ca2+ signaling during the 2448 h post fertilization period of zebrafish development. Thus, it effectively extends the aequorin-based Ca2+-imaging window by an additional 24 hours | Aequorea forskalea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aequorea forskalea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | - |
Aequorea forskalea | ? | - |
? |