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Literature summary for 1.13.12.5 extracted from
- Purification and properties of Renilla reniformis luciferase (1977), Biochemistry, 16, 85-91.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | addition to reaction mixture enhances luciferase activity | Renilla reniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Renilla reniformis |  |
| Cu2+ | - |
Renilla reniformis | |
| Fe2+ | - |
Renilla reniformis | |
| Mn2+ | - |
Renilla reniformis | |
| oxyluciferin | competitive | Renilla reniformis | |
| p-Benzyloxyaniline | - |
Renilla reniformis | |
| Sr2+ | - |
Renilla reniformis | |
| Zn2+ | - |
Renilla reniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulation, optimum at 0.5 M | Renilla reniformis | |
| NaCl | stimulation, optimum at 0.5 M | Renilla reniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | 38000 | gel filtration | Renilla reniformis |
| 33000 | 38000 | sedimentation equilibrium, sedimentation velocity, SDS-PAGE | Renilla reniformis |
| 37000 | - |
1 * 37000, SDS-PAGE, amino acid composition | Renilla reniformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Renilla luciferin + O2 | Renilla reniformis | - |
oxidized Renilla luciferin + CO2 + hv | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Renilla reniformis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 3% carbohydrate by weight, glucosamine and galactosamine | Renilla reniformis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Renilla reniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Renilla reniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-luciferin + O2 + ATP | - |
Renilla reniformis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
| Renilla luciferin + O2 | - |
Renilla reniformis | oxidized Renilla luciferin + CO2 + hv | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, SDS-PAGE, amino acid composition | Renilla reniformis |
| More | tendency to self-association, forming inactive dimers and higher molecular weight species | Renilla reniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Renilla reniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
1 h, stable | Renilla reniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.85 | - |
Luciferin | - |
Renilla reniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Renilla reniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Renilla reniformis |
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