Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces nogalater |
Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces nogalater |
Protein Variants | Comment | Organism |
---|---|---|
H49A | kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 13% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag | Streptomyces nogalater |
N18A | kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 1.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag | Streptomyces nogalater |
N63A | kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 1.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag | Streptomyces nogalater |
R90E | kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 17% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag | Streptomyces nogalater |
W67F | kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 0.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag | Streptomyces nogalater |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 0.1 mM, 9% inhibition | Streptomyces nogalater | |
CoSO4 | 0.1 mM, 10% inhibition | Streptomyces nogalater | |
CuSO4 | 0.1 mM, 99% inhibition | Streptomyces nogalater | |
EDTA | 1 mM, 8% inhibition | Streptomyces nogalater | |
FeSO4 | 0.1 mM, 31% inhibition | Streptomyces nogalater | |
MgSO4 | 0.1 mM, 7% inhibition | Streptomyces nogalater |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme N63A | Streptomyces nogalater | |
0.094 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, wild-type enzyme, recombinant enzyme with N-terminal polyhistidine tag | Streptomyces nogalater | |
0.12 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme N18A | Streptomyces nogalater | |
0.133 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme R90E | Streptomyces nogalater | |
0.14 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, wild-type enzyme, recombinant enzyme construct with a His tag at the C-terminus | Streptomyces nogalater | |
0.15 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme H49A | Streptomyces nogalater | |
0.22 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme W67F | Streptomyces nogalater |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate + O2 | Streptomyces nogalater | the enzyme is involved in the biosynthesis of the aromatic polyketide nogalamycin | [4,5-dihydroxy-9,10-dioxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces nogalater | O54259 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces nogalater |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
deoxynogalonate + O2 = nogalonate + H2O | carbanion mechanism where the deprotonated substrate reacts with molecular oxygen via one electron transfer and formation of a caged radical | Streptomyces nogalater |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate + O2 | - |
Streptomyces nogalater | [4,5-dihydroxy-9,10-dioxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate + H2O | - |
? | |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate + O2 | the enzyme is involved in the biosynthesis of the aromatic polyketide nogalamycin | Streptomyces nogalater | [4,5-dihydroxy-9,10-dioxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SnoaB | - |
Streptomyces nogalater |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Streptomyces nogalater |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme N63A | Streptomyces nogalater | |
0.02 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme W67F | Streptomyces nogalater | |
0.03 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme N18A | Streptomyces nogalater | |
0.3 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme H49A | Streptomyces nogalater | |
0.3 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme R90E | Streptomyces nogalater | |
1.4 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, wild-type enzyme, recombinant enzyme with N-terminal polyhistidine tag | Streptomyces nogalater | |
1.5 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, wild-type enzyme, recombinant enzyme construct with a His tag at the C-terminus | Streptomyces nogalater |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces nogalater |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor detected | Streptomyces nogalater |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the aromatic polyketide nogalamycin | Streptomyces nogalater |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme W67F | Streptomyces nogalater | |
0.22 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme N18A | Streptomyces nogalater | |
0.23 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme N63A | Streptomyces nogalater | |
2 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme H49A | Streptomyces nogalater | |
2.5 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, mutant enzyme R90E | Streptomyces nogalater | |
11 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, wild-type enzyme, recombinant enzyme construct with a His tag at the C-terminus | Streptomyces nogalater | |
15 | - |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate | pH 7.0, 22°C, wild-type enzyme, recombinant enzyme with N-terminal polyhistidine tag | Streptomyces nogalater |