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Literature summary for 1.13.12.21 extracted from
- Tetracenomycin F1 monooxygenase: oxidation of a naphthacenone to a naphthacenequinone in the biosynthesis of tetracenomycin C in Streptomyces glaucescens (1993), Biochemistry, 32, 6656-6663.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 31% loss of activity | Streptomyces glaucescens |  |
| diethyl dicarbonate | - |
Streptomyces glaucescens | |
| N-ethylmaleimide | 0.025 mM, complete inhibition | Streptomyces glaucescens | |
| p-chloromercuribenzoate | 0.025 mM, complete inhibition | Streptomyces glaucescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00747 | - |
tetracenomycin F1 | pH 7.5, 30°C | Streptomyces glaucescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | does not use a metal ion | Streptomyces glaucescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12500 | - |
3 * 12500, SDS-PAGE | Streptomyces glaucescens |
| 37500 | - |
gel filtration | Streptomyces glaucescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetracenomycin F1 + O2 | Streptomyces glaucescens | the enzyme is involved in the biosynthesis of tetracenomycin C | tetracenomycin D3 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces glaucescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces glaucescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.599 | - |
pH 7.5, 30°C | Streptomyces glaucescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetracenomycin F1 + O2 | the enzyme is involved in the biosynthesis of tetracenomycin C | Streptomyces glaucescens | tetracenomycin D3 + H2O | - |
? | |
| tetracenomycin F1 + O2 | i.e. 3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate = 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-2-naphthacenecarboxylic acid | Streptomyces glaucescens | tetracenomycin D3 + H2O | i.e. 3,8,10,12-tetrahydroxy-1-methyl-11-oxo-6,11-dihydro-2-tetracenecarboxylate, i.e. 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-11-oxo-2-naphthacenecarboxylic acid | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 12500, SDS-PAGE | Streptomyces glaucescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TcmF1 monooxygenase | - |
Streptomyces glaucescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces glaucescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | pH 7.0: about 40% of maximal activity, pH 8.0: about 85% of maximal activity | Streptomyces glaucescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | does not possess a prosthetic group such as flavin or heme | Streptomyces glaucescens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of tetracenomycin C | Streptomyces glaucescens |
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