Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | at low lysine concentrations, inhibition by a variety of buffers | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, SDS-PAGE | Pseudomonas fluorescens |
246000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C, 6 months | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 | monooxygenase activity | Pseudomonas fluorescens | 5-aminopentanamide + CO2 + H2O | - |
? | |
additional information | enzyme specific for O2 as electron acceptor, no dehydrogenase activity | Pseudomonas fluorescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 61000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | at low L-lysine concentration | Pseudomonas fluorescens |
9.5 | - |
at saturating L-lysine concentration, 37 mM lysine | Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9.4 | at pH 8.4 about 50% of activity maximum, at pH 9.4 about 40% of activity maximum | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 4 FAD prosthetic groups per enzyme | Pseudomonas fluorescens |