Literature summary for 1.13.12.19 extracted from

Fukuda, H.; Ogawa, T.; Tazaki, M.; Nagahama, K.; Fujii, T.; Tanase, S.; Morino, Y.
Two reactions are simultaneously catalyzed by a single enzyme: The arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae (1992), Biochem. Biophys. Res. Commun., 188, 483-489.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	at the active site, enzyme acts as a bidentate ligand and it forms a complex with Fe2+. The Fe2+ is further coordinated to a tridentate Schiff base of 2-oxoglutarate and L-arginine, whose terminal carboxylate and guanidino groups are trapped by binding sites I and II on the enzyme, respectively	Pseudomonas syringae

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas syringae	P32021	pv. phaseolicola PK2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + O2	presence of oxygen is essential for the ethylene forming reaction by EFE	Pseudomonas syringae	ethylene + ?	-	?
additional information	enzyme catalyzes the formation of ethylene and succinate from 2-oxoglutarate, at a molar ratio of 2:l, reactions of EC 1.13.12.19 and EC 1.14.11.34. In the main reaction, 2-oxoglutarate is dioxygenated to produce one molecule of ethylene and three molecules of carbon dioxide. In the sub-reaction, both 2-oxoglutarate and L-arginine are mono-oxygenated to yield succinate plus carbon dioxide and L-hydroxyarginine, respectively, the latter being further transformed to guanidine and L-delta-pyrroline-5-carboxylate. Dual-circuit mechanism for the entire reaction is proposed, in which the binding of L-arginine and 2-oxoglutarate in a Schiff-base structure generates a common intermediate for two reactions	Pseudomonas syringae	?	-	?

Synonyms

Synonyms	Comment	Organism
EFE	-	Pseudomonas syringae
ethylene-forming enzyme	-	Pseudomonas syringae

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Release 2023.1 (January 2023)