Literature summary for 1.13.12.18 extracted from

Liu, L.; Im, H.; Cegielski, M.; LeMagueres, P.; Schultz, L.W.; Krause, K.L.; Hastings, J.W.
Characterization and crystallization of active domains of a novel luciferase from a marine dinoflagellate (2003), Acta Crystallogr. Sect. D, 59, 761-764.

Search for more literature for 1.13.12.18

Cloned(Commentary)

Cloned (Comment)	Organism
domains D2-LCF and D3-LCF are expressed in Escherichia coli	Lingulodinium polyedra

Crystallization (Commentary)

Crystallization (Comment)	Organism
domains D2-LCF and D3-LCF, sitting drop vapor diffusion method, using 13% (w/v) PEG 10000, 0.075 M bicine pH 7.7, 25% (v/v) glycerol for domain D2-LCF and 18-20% methyl ether PEG 2000, 100 mM EPPS buffer pH 7.8-8.4 for domain D3-LCF	Lingulodinium polyedra

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
scintillon	-	Lingulodinium polyedra	36007	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	2 * 44000, SDS-PAGE	Lingulodinium polyedra
137000	-	calculated from amino acid sequence	Lingulodinium polyedra

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dinoflagellate luciferin + O2	Lingulodinium polyedra	-	oxidized dinoflagellate luciferin + H2O + hv	-	?

Organism

Organism	UniProt	Comment	Textmining
Lingulodinium polyedra	-	formerly Gonyaulax polyedra	-

Purification (Commentary)

Purification (Comment)	Organism
domain D2-LCF and D3-LCF	Lingulodinium polyedra

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dinoflagellate luciferin + O2	-	Lingulodinium polyedra	oxidized dinoflagellate luciferin + H2O + hv	-	?

Subunits

Subunits	Comment	Organism
homotrimer	2 * 44000, SDS-PAGE	Lingulodinium polyedra

Synonyms

Synonyms	Comment	Organism
D2-LCF	second luciferase domain	Lingulodinium polyedra
D3-LCF	third luciferase domain	Lingulodinium polyedra

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Release 2023.1 (January 2023)