Cloned (Comment) | Organism |
---|---|
domains D2-LCF and D3-LCF are expressed in Escherichia coli | Lingulodinium polyedra |
Crystallization (Comment) | Organism |
---|---|
domains D2-LCF and D3-LCF, sitting drop vapor diffusion method, using 13% (w/v) PEG 10000, 0.075 M bicine pH 7.7, 25% (v/v) glycerol for domain D2-LCF and 18-20% methyl ether PEG 2000, 100 mM EPPS buffer pH 7.8-8.4 for domain D3-LCF | Lingulodinium polyedra |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
scintillon | - |
Lingulodinium polyedra | 36007 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Lingulodinium polyedra |
137000 | - |
calculated from amino acid sequence | Lingulodinium polyedra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dinoflagellate luciferin + O2 | Lingulodinium polyedra | - |
oxidized dinoflagellate luciferin + H2O + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lingulodinium polyedra | - |
formerly Gonyaulax polyedra | - |
Purification (Comment) | Organism |
---|---|
domain D2-LCF and D3-LCF | Lingulodinium polyedra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | oxidized dinoflagellate luciferin + H2O + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 2 * 44000, SDS-PAGE | Lingulodinium polyedra |
Synonyms | Comment | Organism |
---|---|---|
D2-LCF | second luciferase domain | Lingulodinium polyedra |
D3-LCF | third luciferase domain | Lingulodinium polyedra |