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Literature summary for 1.13.12.16 extracted from

  • Li, Y.; Gao, Z.; Hou, H.; Li, L.; Zhang, J.; Yang, H.; Dong, Y.; Tan, H.
    Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes (2011), Biochem. Biophys. Res. Commun., 405, 344-348.
    View publication on PubMed
Search for more literature for 1.13.12.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces ansochromogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of NAO from Streptomyces ansochromogenes is determined. It consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern Streptomyces ansochromogenes

Protein Variants

Protein Variants Comment Organism
H179D mutant enzyme shows no activity. Crystal structure of mutant H179D is determined: The structural superimposition of wild-type Sa-NAO and mutant H179D-nitroethane shows no significant structural variation Streptomyces ansochromogenes
H179K mutant enzyme shows no activity Streptomyces ansochromogenes
H179V mutant enzyme shows no activity Streptomyces ansochromogenes

Organism

Organism UniProt Comment Textmining
Streptomyces ansochromogenes Q9FDD4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitropropane + O2
-
 Streptomyces ansochromogenes propionaldehyde + HNO2
-
 ?
2-nitropropane + O2
-
 Streptomyces ansochromogenes acetone + HNO2
-
 ?
nitroethane + O2
-
 Streptomyces ansochromogenes acetaldehyde + HNO2
-
 ?

Synonyms

Synonyms Comment Organism
nitroalkane oxidase
-
 Streptomyces ansochromogenes
Sa-NAO
-
 Streptomyces ansochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces ansochromogenes