Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
H196N | the H196N variant form of the enzyme does to catalyze the formation of ethylnitronate from nitroethane. The H196N variant is a better catalyst than the wild-type enzyme for oxidative turnover with ethylnitronate | Neurospora crassa |
H196N | site-directed mutagenesis, does to catalyze the formation of ethylnitronate from nitroethane. It is a better catalyst than the wild-type enzyme for oxidative turnover with ethylnitronate | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethylnitronate | - |
Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for O2 (wild-type enzyme) is below 0.005 mM | Neurospora crassa | |
0.005 | - |
O2 | 30°C, pH 8, wild-type enzyme, ethylnitronate as a substrate | Neurospora crassa | |
0.034 | - |
O2 | 30°C, pH 8, mutant enzyme H196N | Neurospora crassa | |
0.034 | - |
O2 | 30°C, pH 8, mutant enzyme H196N, ethylnitronate as a substrate | Neurospora crassa | |
11 | - |
ethylnitronate | 30°C, pH 9.5, mutant enzyme H196N | Neurospora crassa | |
15.9 | - |
ethylnitronate | 30°C, pH 9.5, wild-type enzyme | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl nitronate + O2 | - |
Neurospora crassa | ? + nitrite | - |
? | |
additional information | 2-nitropropane dioxygenase utilizes a branched catalytic mechanism with nitroethane as substrate. The branch point occurs at the enzyme-ethylnitronate complex and involves either the release of the nitronate or an oxidative denitrification reaction. The partitioning of the enzyme-nitronate complex results in the formation of multiple products from independent catalytic pathways with nitroethane as substrate for the enzyme. In the nonoxidative pathway, nitroethane is deprotonated by histidine 196 to generate ethylnitronate which is subsequently released from the enzyme as a reaction product | Neurospora crassa | ? | - |
? | |
nitroethane + O2 | 2-nitropropane dioxygenase utilizes a branched catalytic mechanism with nitroethane as substrate. The branch point occurs at the enzyme-ethylnitronate complex and involves either the release of the nitronate or an oxidative denitrification reaction. The partitioning of the enzyme-nitronate complex results in the formation of multiple products from independent catalytic pathways with nitroethane as substrate for the enzyme. In the nonoxidative pathway, nitroethane is deprotonated by histidine 196 to generate ethylnitronate which is subsequently released from the enzyme as a reaction product. The oxidative denitrification pathway was established in previous studies of the enzyme and involves the oxidation of ethylnitronate by the enzyme bound flavin to generate acetaldehyde and nitrite as product | Neurospora crassa | acetaldehyde + nitrite | - |
? | |
nitroethane + O2 | 2-nitropropane dioxygenase utilizes a branched catalytic mechanism with nitroethane as substrate. The branch point occurs at the enzyme-ethylnitronate complex and involves either the release of the nitronate or an oxidative denitrification reaction. The partitioning of the enzyme-nitronate complex results in the formation of multiple products from independent catalytic pathways with nitroethane as substrate for the enzyme. In the nonoxidative pathway, nitroethane is deprotonated by histidine 196 to generate ethylnitronate which is subsequently released from the enzyme as a reaction product. The oxidative denitrification pathway was established in previous studies of the enzyme and involves the oxidation of ethylnitronate by the enzyme bound flavin to generate acetaldehyde and nitrite as product | Neurospora crassa | ethylnitronate | - |
r | |
nitroethane + O2 | - |
Neurospora crassa | ? + nitrite | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-nitropropane dioxygenase | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.2 | - |
ethylnitronate | 30°C, pH 9.5, wild-type enzyme | Neurospora crassa | |
185 | - |
ethylnitronate | 30°C, pH 9.5, mutant enzyme H196N | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | assays are carried out at pH 6, 8, 9.5, and 10 | Neurospora crassa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
ethylnitronate | 30°C, pH 9.5, mutant enzyme H196N | Neurospora crassa | |
100 | - |
ethylnitronate | 30°C, pH 9.5, wild-type enzyme | Neurospora crassa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
O2 | 30°C, pH 8, wild-type enzyme | Neurospora crassa | |
0.0055 | - |
O2 | 30°C, pH 8, mutant enzyme H196N | Neurospora crassa | |
1.02 | - |
ethylnitronate | 30°C, pH 8, wild-type enzyme | Neurospora crassa | |
16.8 | - |
ethylnitronate | 30°C, pH 8, mutant enzyme H196N | Neurospora crassa |