Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(3,4-dihydroxyphenyl)-L-Ala | - |
Cyberlindnera mrakii | |
5-hydroxytryptophan | - |
Cyberlindnera mrakii | |
acetone | inhibits competitively against 2-nitropropane, no inhibitory effect against O2 | Cyberlindnera mrakii | |
alpha-Naphthol | - |
Cyberlindnera mrakii | |
catechol | - |
Cyberlindnera mrakii | |
cytochrome c | competitive inhibitor | Cyberlindnera mrakii | |
epinephrine | competitive inhibitor | Cyberlindnera mrakii | |
hydroquinone | - |
Cyberlindnera mrakii | |
additional information | inhibition by superoxide dismutase and various scavengers for superoxide; phenol, tryptophan, and tryptamine show no effect on the reaction | Cyberlindnera mrakii | |
NADH | competitive inhibitor. Oxygenation is inhibited approximately 86% by 0.1 mM NADH, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized | Cyberlindnera mrakii | |
NADPH | competitive inhibitor. oxygenation is inhibited approximately 100% by 0.1 mM NADPH, respectively, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized | Cyberlindnera mrakii | |
nitrite | inhibits noncompetitively against 2-nitropropane, functioned as a noncompetitive inhibitor against oxygen in the presence of excess 2-nitropropane (50 mM) | Cyberlindnera mrakii | |
phloroglucinol | - |
Cyberlindnera mrakii | |
pyrogallol | - |
Cyberlindnera mrakii | |
resorcinol | - |
Cyberlindnera mrakii | |
serotonin | - |
Cyberlindnera mrakii | |
Superoxide dismutase | - |
Cyberlindnera mrakii | |
Thiophenol | - |
Cyberlindnera mrakii | |
Tiron | i.e. pyrocatechol-3,5-disulfonate disodium salt | Cyberlindnera mrakii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.277 | - |
O2 | - |
Cyberlindnera mrakii | |
19.2 | - |
2-Nitropropane | - |
Cyberlindnera mrakii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera mrakii | - |
- |
- |
Cyberlindnera mrakii | - |
IF0 0895 | - |
Cyberlindnera mrakii IF0 0895 | - |
IF0 0895 | - |
Purification (Comment) | Organism |
---|---|
purified to homogeneity | Cyberlindnera mrakii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ethylnitronate + O2 = acetaldehyde + nitrite + other products | superoxide as reactive intermediate | Cyberlindnera mrakii | |
ethylnitronate + O2 = acetaldehyde + nitrite + other products | ordered bi bi mechanism in which 2-nitropropane first combines with the enzyme and the enzyme 2-nitropropane complex reacts with oxygen to form a ternary complex | Cyberlindnera mrakii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitropropane + O2 | superoxide as reactive intermediate | Cyberlindnera mrakii | acetone + HNO2 | - |
? | |
2-nitropropane + O2 | superoxide as reactive intermediate | Cyberlindnera mrakii IF0 0895 | acetone + HNO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-nitropropane dioxygenase | - |
Cyberlindnera mrakii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | cytochrome c also inhibits the reaction and half-inhibition is found in the presence of 17 microM cytochrome c. The addition of cytochrome c to the reaction mixture containing 2-nitropropane and 2-nitropropane dioxygenase causes an increase in absorbance at 550 nm indicating the reduction of cytochrome c | Cyberlindnera mrakii |