Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Cereibacter sphaeroides | |
NADH | - |
Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for 3,4-dihydroxy-L-phenylalanine is 11.84 microM | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
gel filtration | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Cereibacter sphaeroides OU5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxy-L-phenylalanine + O2 | - |
Cereibacter sphaeroides | 3,4-dihydroxyphenylpyruvate + NH3 | - |
? | |
3,4-dihydroxy-L-phenylalanine + O2 | - |
Cereibacter sphaeroides OU5 | 3,4-dihydroxyphenylpyruvate + NH3 | - |
? | |
glycine + O2 | - |
Cereibacter sphaeroides | oxoacetic acid + NH3 | 30% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
glycine + O2 | - |
Cereibacter sphaeroides OU5 | oxoacetic acid + NH3 | 30% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-alanine + O2 | - |
Cereibacter sphaeroides | 2-oxo-propanoic acid + NH3 | 20% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-alanine + O2 | - |
Cereibacter sphaeroides OU5 | 2-oxo-propanoic acid + NH3 | 20% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-glutamate + O2 | - |
Cereibacter sphaeroides | 2-oxopentanedioic acid + NH3 | 60% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-glutamate + O2 | - |
Cereibacter sphaeroides OU5 | 2-oxopentanedioic acid + NH3 | 60% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-phenylalanine + H2O + O2 | - |
Cereibacter sphaeroides | 2-oxo-3-phenylpropanoic acid + NH3 | 60% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-phenylalanine + H2O + O2 | - |
Cereibacter sphaeroides OU5 | 2-oxo-3-phenylpropanoic acid + NH3 | 60% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-tryptophan + O2 | - |
Cereibacter sphaeroides | 3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 | 50% of the activity with 3,4-dihydroxy-L-phenylalanine | ? | |
L-tyrosine + O2 | - |
Cereibacter sphaeroides | 3-(4-hydroxyphenyl)-2-oxopropanoic acid + NH3 | 80% of the activity with 3,4-dihydroxy-L-phenylalanine | ? |
Subunits | Comment | Organism |
---|---|---|
pentamer | five different subunits of 54000 Dalton, 43000 Dalton, 34000 Dalton, 25000 Dalton, and 22000 Dalton, SDS-PAGE | Cereibacter sphaeroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
3,4-dihydroxy-L-phenylalanine | pH 7.8, 30°C | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required | Cereibacter sphaeroides |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 value for NADH is 35 microM per l, IC50 value for 2-oxoglutarate is 25 microM per l | Cereibacter sphaeroides | additional information |