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Literature summary for 1.13.12.13 extracted from

  • Inouye, S.; Sato, J.; Sahara-Miura, Y.; Yoshida, S.; Kurakata, H.; Hosoya, T.
    C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: the mutated catalytic 19 kDa component of Oplophorus luciferase (2013), Biochem. Biophys. Res. Commun., 437, 23-28.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the fused proteinof nanoKAZ with IgG-binding domain (ZZ domain) is expressed in the cytoplasm of Escherichia coli cells as a soluble form and purified. A secretory expression of nanoKAZ in CHO-K1 cells is performed using nanoKAZ fused with the signal peptide sequence of Gaussia luciferase Oplophorus gracilirostris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
Western blot, expression in CHO cells Oplophorus gracilirostris

Organism

Organism UniProt Comment Textmining
Oplophorus gracilirostris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Oplophorus gracilirostris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6h-coelenterazine + O2 luminescence intensity (Imax): 0.8 Oplophorus gracilirostris oxidized 6h-coelenterazine + CO2 + hnu
-
?
6h-f-coelenterazine + O2 luminescence intensity (Imax): 10.1 Oplophorus gracilirostris oxidized 6h-f-coelenterazine + CO2 + hnu
-
?
bis-coelenterazine + O2 luminescence intensity (Imax): 10.3 Oplophorus gracilirostris oxidized bis-coelenterazine + CO2 + hnu
-
?
coelenterazine + O2 luminescence intensity (Imax): 1.0 Oplophorus gracilirostris oxidized coelenterazine + CO2 + hn
-
?
f-coelenterazine + O2 luminescence intensity (Imax): 19.5 Oplophorus gracilirostris oxidized f-coelenterazine + CO2 + hnu
-
?
furimazine + O2 luminescence intensity (Imax): 10.1 Oplophorus gracilirostris ?
-
?
h-coelenterazine + O2 luminescence intensity (Imax): 17 Oplophorus gracilirostris oxidized h-coelenterazine + CO2 + hnu
-
?

Synonyms

Synonyms Comment Organism
nanoKAZ mutated catalytic component of Oplophorus luciferase Oplophorus gracilirostris
Oplophorus luciferase
-
Oplophorus gracilirostris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Oplophorus gracilirostris