Cloned (Comment) | Organism |
---|---|
the fused proteinof nanoKAZ with IgG-binding domain (ZZ domain) is expressed in the cytoplasm of Escherichia coli cells as a soluble form and purified. A secretory expression of nanoKAZ in CHO-K1 cells is performed using nanoKAZ fused with the signal peptide sequence of Gaussia luciferase | Oplophorus gracilirostris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
Western blot, expression in CHO cells | Oplophorus gracilirostris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oplophorus gracilirostris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Oplophorus gracilirostris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6h-coelenterazine + O2 | luminescence intensity (Imax): 0.8 | Oplophorus gracilirostris | oxidized 6h-coelenterazine + CO2 + hnu | - |
? | |
6h-f-coelenterazine + O2 | luminescence intensity (Imax): 10.1 | Oplophorus gracilirostris | oxidized 6h-f-coelenterazine + CO2 + hnu | - |
? | |
bis-coelenterazine + O2 | luminescence intensity (Imax): 10.3 | Oplophorus gracilirostris | oxidized bis-coelenterazine + CO2 + hnu | - |
? | |
coelenterazine + O2 | luminescence intensity (Imax): 1.0 | Oplophorus gracilirostris | oxidized coelenterazine + CO2 + hn | - |
? | |
f-coelenterazine + O2 | luminescence intensity (Imax): 19.5 | Oplophorus gracilirostris | oxidized f-coelenterazine + CO2 + hnu | - |
? | |
furimazine + O2 | luminescence intensity (Imax): 10.1 | Oplophorus gracilirostris | ? | - |
? | |
h-coelenterazine + O2 | luminescence intensity (Imax): 17 | Oplophorus gracilirostris | oxidized h-coelenterazine + CO2 + hnu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nanoKAZ | mutated catalytic component of Oplophorus luciferase | Oplophorus gracilirostris |
Oplophorus luciferase | - |
Oplophorus gracilirostris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Oplophorus gracilirostris |