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Literature summary for 1.13.11.B6 extracted from

  • Veronico, P.; Giannino, D.; Melillo, M.T.; Leone, A.; Reyes, A.; Kennedy, M.W.; Bleve-Zacheo, T.
    A novel lipoxygenase in pea roots. Its function in wounding and biotic stress (2006), Plant Physiol., 141, 1045-1055.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
x * 94000, SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum Q14ST9 subsp. transcaucasicum Govorov
-

Source Tissue

Source Tissue Comment Organism Textmining
root LOXN2 message is widespread in the cortex and endodermis of healthy roots, but specifically localized at high level in the cells bordering the nematode-induced syncytia of infected roots. In uninfected roots, the transcript is visualized in cortical and endodermal cells. In resistant infected roots, faint and infrequent signal spots are observed in the cortex, but LOXN2 message is abundant in the outer cells surrounding the vascular cylinder, in those cells injured by nematode penetration and those flanking the induced syncytia. In susceptible infected roots, the transcript is detected in cortical cells damaged by nematode invasion and undergoing necrosis, and in parenchymatous cells of the vascular cylinder strictly related to syncytia development Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.35
-
activity with linoleate Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2 specific activity is 13% of the activity with linoleate Pisum sativum ? products not determined ?
linoleate + O2
-
Pisum sativum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate the ratio of 9-hydroperoxy-(10E,12Z)-octadecadienoate to 13-hydroperoxy-(10E,12Z)-octadecadienoate is 3:1 ?
linolenate + O2 specific activity is 17% of the activity with linoleate. The R/S stereoconfiguration of the product is not determined Pisum sativum ? products not determined ?

Subunits

Subunits Comment Organism
? x * 94000, SDS-PAGE Pisum sativum

Synonyms

Synonyms Comment Organism
9-/13-LOXN2
-
Pisum sativum

Expression

Organism Comment Expression
Pisum sativum LOXN2 transcription is monitored in roots after mechanical injury and during nematode infection. The message peaks at 3 and 24 h after wounding in both genotypes and is more abundant in the resistant than in the susceptible pea. In nematode-infected roots, transcription of several LOX genes is triggered except LOXN2, which is repressed in both genotypes down

General Information

General Information Comment Organism
physiological function LOXN2 may be involved in late mechanisms of host resistance Pisum sativum