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Literature summary for 1.13.11.B6 extracted from

  • Palmieri-Thiers, C.; Canaan, S.; Brunini, V.; Lorenzi, V.; Tomi, F.; Desseyn, J.L.; Garscha, U.; Oliw, E.H.; Berti, L.; Maury, J.
    A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening (2009), Biochim. Biophys. Acta, 1791, 339-346.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Olea europaea

Inhibitors

Inhibitors Comment Organism Structure
nordihydroguaiaretic acid noncompetitive Olea europaea
propyl gallate competitive Olea europaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.175
-
linoleate 25°C Olea europaea
0.344
-
linolenate 25°C Olea europaea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol likely confined to the cytosol, because it contains neither consensus targeting nor retention signals for any organelles Olea europaea 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
x * 98000, SDS-PAGE Olea europaea
98400
-
x * 98400, calculated from sequence Olea europaea

Organism

Organism UniProt Comment Textmining
Olea europaea B6D1W5 L., Leccino variety
-

Purification (Commentary)

Purification (Comment) Organism
-
Olea europaea

Source Tissue

Source Tissue Comment Organism Textmining
fruit detected in all olive developmental and ripening stages from green small fruits to black or senescent fruits. Mainly expressed at late developmental stages Olea europaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.4
-
-
Olea europaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2 the catalytic efficiency of the recombinant olive LOX was significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation Olea europaea (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate dual positional specificity: the enzyme forms (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate in a 2:1 ratio, the products are predominantly in 9S and 13R configuration ?
linolenate + O2 the catalytic efficiency of the recombinant olive LOX is significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation Olea europaea ?
-
?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE Olea europaea
? x * 98400, calculated from sequence Olea europaea

Synonyms

Synonyms Comment Organism
9/13-LOX
-
Olea europaea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.8
-
linolenate 25°C Olea europaea
48.6
-
linoleate 25°C Olea europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Olea europaea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00188
-
propyl gallate 25°C Olea europaea
0.00409
-
nordihydroguaiaretic acid 25°C Olea europaea

pI Value

Organism Comment pI Value Maximum pI Value
Olea europaea calculated from sequence
-
5.95

General Information

General Information Comment Organism
physiological function the 9/13-LOX is associated with the ripening and senescence processes. Due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered Olea europaea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
139
-
linolenate 25°C Olea europaea
278
-
linoleate 25°C Olea europaea