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Literature summary for 1.13.11.81 extracted from
- One substrate, five products: reactions catalyzed by the dihydroneopterin aldolase from Mycobacterium tuberculosis (2012), J. Am. Chem. Soc., 134, 19758-19771.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000154 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16585 | - |
4 * 16585, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
| 16716 | - |
4 * 16716, calculated from amino acid sequence | Mycobacterium tuberculosis |
| 62300 | - |
sedimentation equilibrium analysis | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin + O2 | Mycobacterium tuberculosis | - |
7,8-dihydroxanthopterin + formate + glycolaldehyde | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WNC5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin + O2 | - |
Mycobacterium tuberculosis | 7,8-dihydroxanthopterin + formate + glycolaldehyde | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 16585, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
| homotetramer | 4 * 16716, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHNA | - |
Mycobacterium tuberculosis |
| dihydroneopterin oxygenase | - |
Mycobacterium tuberculosis |
| Rv3607c | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.3 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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