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Literature summary for 1.13.11.8 extracted from
- Kinetic and Mossbauer studies on the mechanism of protocatechuic acid 4,5-oxygenase (1972), Biochemistry, 11, 3212-3219.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-nitrocatechol | competitive inhibitor | Comamonas testosteroni |  |
| iodoacetamide | competitive inhibitor | Comamonas testosteroni | |
| p-chloromercuribenzoic acid | excellent competitive inhibitor | Comamonas testosteroni | |
| Protocatechualdehyde | competitive inhibitor | Comamonas testosteroni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
protocatechuate | - |
Comamonas testosteroni | |
| 0.0735 | - |
gallic acid | - |
Comamonas testosteroni | |
| 0.125 | - |
5-methoxygallic acid | - |
Comamonas testosteroni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | four iron atoms per molecule | Comamonas testosteroni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
- |
Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protocatechuate + O2 | Comamonas testosteroni | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | - |
grown on p-hydroxybenzoate-containing medium | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde | involvement of sulfhydryl groups at the active site, 2 free sulfhydryl groups per enzyme molecule | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methoxygallic acid + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| gallic acid + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| protocatechuate + O2 | - |
Comamonas testosteroni | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme assay | Comamonas testosteroni |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
4-nitrocatechol | - |
Comamonas testosteroni | |
| 0.96 | - |
Protocatechualdehyde | - |
Comamonas testosteroni | |
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