Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | complete inhibition at 1 mM | Pseudomonas sp. | |
EDTA | 99% inhibition at 5 mM | Pseudomonas sp. | |
Fe3+ | complete inhibition at 1 mM | Pseudomonas sp. | |
Mg2+ | concentrations of Mg2+ higher than 1.25 mM inhibit activity strongly (90% of activity lost at 1.75 mM) | Pseudomonas sp. | |
additional information | not inhibited by 5 mM KCN | Pseudomonas sp. | |
oleate | at concentrations of oleate higher than 2.1 mM, the enzyme activity is inhibited by excess of substrate | Pseudomonas sp. | |
Zn2+ | complete inhibition at 1 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
linoleate | in 0.2 M sodium borate buffer, pH 9.0, at 30°C | Pseudomonas sp. | |
0.73 | - |
alpha-linolenate | in 0.2 M sodium borate buffer, pH 9.0, at 30°C | Pseudomonas sp. | |
0.74 | - |
oleate | in 0.2 M sodium borate buffer, pH 9.0, at 30°C | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains 0.55 mol of Fe2+ per mol of protein | Pseudomonas sp. | |
Mg2+ | the presence of 1 mM Mg2+ increases enzyme activity 1.5fold | Pseudomonas sp. | |
Mn2+ | the enzyme contains 25 mmol Mn2+ per mol of protein | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | Pseudomonas sp. | 100% activity | (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate | - |
? | |
linoleate + O2 | Pseudomonas sp. 42A2 | 100% activity | (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate | - |
? | |
oleate + O2 | Pseudomonas sp. | 46% activity compared to linoleate | (8E,10S)-10-hydroperoxyoctadeca-8-enoate | - |
? | |
oleate + O2 | Pseudomonas sp. 42A2 | 46% activity compared to linoleate | (8E,10S)-10-hydroperoxyoctadeca-8-enoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 42A2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, 1 week, no loss of activity (thawing and freezing decreases enzyme activity by 30%) | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenate + O2 | 60.4% activity compared to linoleate | Pseudomonas sp. | (8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate | - |
? | |
alpha-linolenate + O2 | 60.4% activity compared to linoleate | Pseudomonas sp. 42A2 | (8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate | - |
? | |
arachidonate + O2 | 0.9% activity compared to linoleate | Pseudomonas sp. | ? | - |
? | |
arachidonate + O2 | 0.9% activity compared to linoleate | Pseudomonas sp. 42A2 | ? | - |
? | |
cis-12-octadecenoate + O2 | 8.9% activity compared to linoleate | Pseudomonas sp. | ? | - |
? | |
linoleate + O2 | 100% activity | Pseudomonas sp. | (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate | - |
? | |
linoleate + O2 | 100% activity | Pseudomonas sp. 42A2 | (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate | - |
? | |
additional information | the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids | Pseudomonas sp. | ? | - |
? | |
additional information | the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids | Pseudomonas sp. 42A2 | ? | - |
? | |
oleate + O2 | 46% activity compared to linoleate | Pseudomonas sp. | (8E,10S)-10-hydroperoxyoctadeca-8-enoate | - |
? | |
oleate + O2 | 46% activity compared to linoleate | Pseudomonas sp. 42A2 | (8E,10S)-10-hydroperoxyoctadeca-8-enoate | - |
? | |
petroselinoate + O2 | 26.4% activity compared to linoleate | Pseudomonas sp. | ? | - |
? | |
triolein + O2 | 0.4% activity compared to linoleate | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
LOX | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | 44% of activity still remains at 55°C. The enzyme is stable up to 45 °C, but sharply decreases after 50°C. Activity is completely lost at 70°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Pseudomonas sp. |