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Literature summary for 1.13.11.77 extracted from

  • Busquets, M.; Deroncelé, V.; Vidal-Mas, J.; Rodríguez, E.; Guerrero, A.; Manresa, A.
    Isolation and characterization of a lipoxygenase from Pseudomonas 42A2 responsible for the biotransformation of oleic acid into (S)-(E)-10-hydroxy-8-octadecenoic acid (2004), Antonie van Leeuwenhoek, 85, 129-139.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ complete inhibition at 1 mM Pseudomonas sp.
EDTA 99% inhibition at 5 mM Pseudomonas sp.
Fe3+ complete inhibition at 1 mM Pseudomonas sp.
Mg2+ concentrations of Mg2+ higher than 1.25 mM inhibit activity strongly (90% of activity lost at 1.75 mM) Pseudomonas sp.
additional information not inhibited by 5 mM KCN Pseudomonas sp.
oleate at concentrations of oleate higher than 2.1 mM, the enzyme activity is inhibited by excess of substrate Pseudomonas sp.
Zn2+ complete inhibition at 1 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
linoleate in 0.2 M sodium borate buffer, pH 9.0, at 30°C Pseudomonas sp.
0.73
-
alpha-linolenate in 0.2 M sodium borate buffer, pH 9.0, at 30°C Pseudomonas sp.
0.74
-
oleate in 0.2 M sodium borate buffer, pH 9.0, at 30°C Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains 0.55 mol of Fe2+ per mol of protein Pseudomonas sp.
Mg2+ the presence of 1 mM Mg2+ increases enzyme activity 1.5fold Pseudomonas sp.
Mn2+ the enzyme contains 25 mmol Mn2+ per mol of protein Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Pseudomonas sp. 100% activity (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
?
linoleate + O2 Pseudomonas sp. 42A2 100% activity (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
?
oleate + O2 Pseudomonas sp. 46% activity compared to linoleate (8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
?
oleate + O2 Pseudomonas sp. 42A2 46% activity compared to linoleate (8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 42A2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration Pseudomonas sp.

Storage Stability

Storage Stability Organism
4°C, 1 week, no loss of activity (thawing and freezing decreases enzyme activity by 30%) Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenate + O2 60.4% activity compared to linoleate Pseudomonas sp. (8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
?
alpha-linolenate + O2 60.4% activity compared to linoleate Pseudomonas sp. 42A2 (8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
?
arachidonate + O2 0.9% activity compared to linoleate Pseudomonas sp. ?
-
?
arachidonate + O2 0.9% activity compared to linoleate Pseudomonas sp. 42A2 ?
-
?
cis-12-octadecenoate + O2 8.9% activity compared to linoleate Pseudomonas sp. ?
-
?
linoleate + O2 100% activity Pseudomonas sp. (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
?
linoleate + O2 100% activity Pseudomonas sp. 42A2 (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
?
additional information the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids Pseudomonas sp. ?
-
?
additional information the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids Pseudomonas sp. 42A2 ?
-
?
oleate + O2 46% activity compared to linoleate Pseudomonas sp. (8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
?
oleate + O2 46% activity compared to linoleate Pseudomonas sp. 42A2 (8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
?
petroselinoate + O2 26.4% activity compared to linoleate Pseudomonas sp. ?
-
?
triolein + O2 0.4% activity compared to linoleate Pseudomonas sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
LOX
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 70 44% of activity still remains at 55°C. The enzyme is stable up to 45 °C, but sharply decreases after 50°C. Activity is completely lost at 70°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Pseudomonas sp.