Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 79.4% residual activity at 2 mM | Comamonas testosteroni | |
Cd2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
Co2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
Cu2+ | 2.9% residual activity at 2 mM | Comamonas testosteroni | |
EDTA | - |
Comamonas testosteroni | |
Fe2+ | 57.4% residual activity at 2 mM | Comamonas testosteroni | |
Fe3+ | 4.4% residual activity at 2 mM | Comamonas testosteroni | |
H2O2 | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
K3Fe(CN)6 | 41.2% residual activity at 2 mM | Comamonas testosteroni | |
Mg2+ | 91.2% residual activity at 2 mM | Comamonas testosteroni | |
Mn2+ | 30.9% residual activity at 2 mM | Comamonas testosteroni | |
Ni2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
Zn2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
2-amino-5-chlorophenol | in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C | Comamonas testosteroni | |
0.00089 | - |
2-Aminophenol | in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 38000 + 2 * 33000, SDS-PAGE | Comamonas testosteroni |
38000 | - |
2 * 38000 + 2 * 33000, SDS-PAGE | Comamonas testosteroni |
130000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-chlorophenol + O2 | Comamonas testosteroni | 68% activity compared to 2-aminophenol | ? | - |
? | |
2-amino-5-chlorophenol + O2 | Comamonas testosteroni CNB-1 | 68% activity compared to 2-aminophenol | ? | - |
? | |
2-aminophenol + O2 | Comamonas testosteroni | 100% activity | 2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | Comamonas testosteroni CNB-1 | 100% activity | 2-aminomuconate 6-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q6J1Z5 and Q6J1Z6 | subunit alpha and catalytic subunit beta | - |
Comamonas testosteroni CNB-1 | Q6J1Z5 and Q6J1Z6 | subunit alpha and catalytic subunit beta | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 200 gel filtration | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-chlorophenol + O2 | 68% activity compared to 2-aminophenol | Comamonas testosteroni | ? | - |
? | |
2-amino-5-chlorophenol + O2 | 68% activity compared to 2-aminophenol | Comamonas testosteroni CNB-1 | ? | - |
? | |
2-aminophenol + O2 | 100% activity | Comamonas testosteroni | 2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | 100% activity | Comamonas testosteroni CNB-1 | 2-aminomuconate 6-semialdehyde | - |
? | |
additional information | 5% activity with catechol | Comamonas testosteroni | ? | - |
? | |
additional information | 5% activity with catechol | Comamonas testosteroni CNB-1 | ? | - |
? | |
protocatechuate + O2 | 33% activity compared to 2-aminophenol | Comamonas testosteroni | ? | - |
? | |
protocatechuate + O2 | 33% activity compared to 2-aminophenol | Comamonas testosteroni CNB-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 38000 + 2 * 33000, SDS-PAGE | Comamonas testosteroni |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2 | - |
in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C | Comamonas testosteroni | EDTA |