Crystallization (Comment) | Organism |
---|---|
holoenzyme, with FeII and in complexes with the substrate 2-aminophenol and the suicide inhibitor 4-nitrocatechol, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate pH 6.5, 25% (w/v) PEG 3350, 0.2 M sodium chloride | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrocatechol | suicide inhibitor | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
2 * 35000 + 2 * 29000, calculated from amino acid sequence | Comamonas testosteroni |
30000 | - |
2 * 35000 + 2 * 30000, SDS-PAGE | Comamonas testosteroni |
35000 | - |
2 * 35000 + 2 * 29000, calculated from amino acid sequence | Comamonas testosteroni |
35000 | - |
2 * 35000 + 2 * 30000, SDS-PAGE | Comamonas testosteroni |
140000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminophenol + O2 | Comamonas testosteroni | - |
2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | Comamonas testosteroni CNB-1 | - |
2-aminomuconate 6-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q6J1Z5 and Q6J1Z6 | subunit alpha and catalytic subunit beta | - |
Comamonas testosteroni CNB-1 | Q6J1Z5 and Q6J1Z6 | subunit alpha and catalytic subunit beta | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 200 gel filtration | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminophenol + O2 | - |
Comamonas testosteroni | 2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | - |
Comamonas testosteroni CNB-1 | 2-aminomuconate 6-semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 35000 + 2 * 29000, calculated from amino acid sequence | Comamonas testosteroni |
heterotetramer | 2 * 35000 + 2 * 30000, SDS-PAGE | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
APD | - |
Comamonas testosteroni |