Literature summary for 1.13.11.74 extracted from

Li, d.e..F.; Zhang, J.Y.; Hou, Y.J.; Liu, L.; Hu, Y.; Liu, S.J.; Wang, d.a..C.; Liu, W.
Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor (2013), Acta Crystallogr. Sect. D, 69, 32-43.

Search for more literature for 1.13.11.74

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Comamonas testosteroni

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with (4Z,6Z)-3-iminooxepin-2(3H)-one, 2-aminomuconic 6-semialdehyde and 4-nitrocatechol, hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 mM sodium chloride, 0.1 M sodium cacodylate pH 6.5	Comamonas testosteroni

Protein Variants

Protein Variants	Comment	Organism
E251A	inactive	Comamonas testosteroni
H13A	inactive	Comamonas testosteroni
H195Q	inactive	Comamonas testosteroni
H62A	inactive	Comamonas testosteroni
Y129F	inactive	Comamonas testosteroni

Inhibitors

Inhibitors	Comment	Organism	Structure
4-nitrocatechol	suicide inhibitor, the enzyme completely loses its activity towards 2-aminophenol after incubation with 4-nitrocatechol for 100 s	Comamonas testosteroni
catechol	the enzyme completely loses its activity towards 2-aminophenol after incubation with catechol for 100 s	Comamonas testosteroni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0171	-	2-Aminophenol	at pH 8.0, temperature not specified in the publication	Comamonas testosteroni
0.0775	-	O2	at pH 8.0, temperature not specified in the publication	Comamonas testosteroni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains Fe2+	Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-aminophenol + O2	Comamonas testosteroni	-	2-aminomuconate 6-semialdehyde	-	?
2-aminophenol + O2	Comamonas testosteroni CNB-1	-	2-aminomuconate 6-semialdehyde	-	?

Organism

Organism	UniProt	Comment	Textmining
Comamonas testosteroni	-	-	-
Comamonas testosteroni CNB-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Comamonas testosteroni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-aminophenol + O2	-	Comamonas testosteroni	2-aminomuconate 6-semialdehyde	-	?
2-aminophenol + O2	-	Comamonas testosteroni CNB-1	2-aminomuconate 6-semialdehyde	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	-	Comamonas testosteroni

Synonyms

Synonyms	Comment	Organism
APD	-	Comamonas testosteroni

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Release 2023.1 (January 2023)