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Literature summary for 1.13.11.71 extracted from

  • Schwartz, S.H.; Qin, X.; Loewen, M.C.
    The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching (2004), J. Biol. Chem., 279, 46940-46945.
    View publication on PubMed
Search for more literature for 1.13.11.71

Activating Compound

Activating Compound Comment Organism Structure
methanol addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 9–10 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0152
-
 beta-carotene pH 7.0, temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast protein contains a probable chloroplast targeting sequence of 31 amino acids Arabidopsis thaliana 9507
-

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q7XJM2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
 Arabidopsis thaliana 10'-apo-beta-carotenal + beta-ionone
-
 ?
additional information no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
CCD7
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching Arabidopsis thaliana