Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methanol | addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 910 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0152 | - |
beta-carotene | pH 7.0, temperature not specified in the publication | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | protein contains a probable chloroplast targeting sequence of 31 amino acids | Arabidopsis thaliana | 9507 | - |
Organic Solvent | Comment | Organism |
---|---|---|
Methanol | addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q7XJM2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | - |
Arabidopsis thaliana | 10'-apo-beta-carotenal + beta-ionone | - |
? | |
additional information | no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCD7 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |