Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
beta-apo-8'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis | |
0.0219 | - |
3-hydroxy-beta-apo-8'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis | |
0.0294 | - |
beta-apo-10'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis | |
0.0438 | - |
3-hydroxy-beta-apo-10'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPR5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPR5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-beta-apo-10'-carotenal + O2 | - |
Mycobacterium tuberculosis | 3-hydroxy-beta-apo-13-carotenone + 3-hydroxy-beta-apo-15-carotenal | plus a minor product presumably representing 3-hydroxy-beta-apo-11-carotenal | ? | |
3-hydroxy-beta-apo-8'-carotenal + O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
beta-apo-10'-carotenal + O2 | - |
Mycobacterium tuberculosis | beta-apo-13-carotenone | plus minor amounts of beta-apo-15-carotenal | ? | |
beta-apo-8'-carotenal + O2 | - |
Mycobacterium tuberculosis | beta-apo-13-carotenone + retinal | - |
? | |
beta-carotene + O2 | - |
Mycobacterium tuberculosis | retinal + beta-apo-14'-carotenal + beta-apo-13-carotenone | - |
? | |
lutein + O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
additional information | enzyme cleaves the central C15-C15' and an excentric double bond at the C13-C14 position, leading to retinal (C20), beta-apo-14'-carotenal (C22) and beta-apo-13-carotenone (C18) from beta-carotene, as well as the corresponding hydroxylated products from zeaxanthin and lutein. Enzyme also cleaves 3,3'-dihydroxy-isorenieratene representing aromatic carotenoids synthesized by other mycobacteria. The preference for each of the cleavage positions is determined by the hydroxylation and the nature of the ionone ring. Incubation of apocarotenoids shorter than beta-apo-10'-carotenal reveals only weak activity, substrates with a shorter chain length are not converted | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no substrates: resveratrol, cholecalciferol, phylloquinone and alpha-tocopherol | Mycobacterium tuberculosis | ? | - |
? | |
zeaxanthin + O2 | - |
Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carotenoid cleavage oxygenase | - |
Mycobacterium tuberculosis |
CCO | - |
Mycobacterium tuberculosis |
Rv0654 | - |
Mycobacterium tuberculosis |
Rv0654 | gene locus | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
392.7 | - |
beta-apo-8'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis | |
561.7 | - |
beta-apo-10'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis | |
764.3 | - |
3-hydroxy-beta-apo-10'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis | |
1308 | - |
3-hydroxy-beta-apo-8'-carotenal | pH 7.8, 28°C | Mycobacterium tuberculosis |