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Literature summary for 1.13.11.65 extracted from

  • Scherzinger, D.; Scheffer, E.; Bar, C.; Ernst, H.; Al-Babili, S.
    The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids (2010), FEBS J., 277, 4662-4673.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
0.0219
-
3-hydroxy-beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
0.0294
-
beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
0.0438
-
3-hydroxy-beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPR5
-
-
Mycobacterium tuberculosis H37Rv P9WPR5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-beta-apo-10'-carotenal + O2
-
Mycobacterium tuberculosis 3-hydroxy-beta-apo-13-carotenone + 3-hydroxy-beta-apo-15-carotenal plus a minor product presumably representing 3-hydroxy-beta-apo-11-carotenal ?
3-hydroxy-beta-apo-8'-carotenal + O2
-
Mycobacterium tuberculosis ?
-
?
beta-apo-10'-carotenal + O2
-
Mycobacterium tuberculosis beta-apo-13-carotenone plus minor amounts of beta-apo-15-carotenal ?
beta-apo-8'-carotenal + O2
-
Mycobacterium tuberculosis beta-apo-13-carotenone + retinal
-
?
beta-carotene + O2
-
Mycobacterium tuberculosis retinal + beta-apo-14'-carotenal + beta-apo-13-carotenone
-
?
lutein + O2
-
Mycobacterium tuberculosis ?
-
?
additional information enzyme cleaves the central C15-C15' and an excentric double bond at the C13-C14 position, leading to retinal (C20), beta-apo-14'-carotenal (C22) and beta-apo-13-carotenone (C18) from beta-carotene, as well as the corresponding hydroxylated products from zeaxanthin and lutein. Enzyme also cleaves 3,3'-dihydroxy-isorenieratene representing aromatic carotenoids synthesized by other mycobacteria. The preference for each of the cleavage positions is determined by the hydroxylation and the nature of the ionone ring. Incubation of apocarotenoids shorter than beta-apo-10'-carotenal reveals only weak activity, substrates with a shorter chain length are not converted Mycobacterium tuberculosis ?
-
?
additional information no substrates: resveratrol, cholecalciferol, phylloquinone and alpha-tocopherol Mycobacterium tuberculosis ?
-
?
zeaxanthin + O2
-
Mycobacterium tuberculosis ?
-
?

Synonyms

Synonyms Comment Organism
carotenoid cleavage oxygenase
-
Mycobacterium tuberculosis
CCO
-
Mycobacterium tuberculosis
Rv0654
-
Mycobacterium tuberculosis
Rv0654 gene locus Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
392.7
-
beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
561.7
-
beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
764.3
-
3-hydroxy-beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1308
-
3-hydroxy-beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis