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Literature summary for 1.13.11.64 extracted from

  • Qu, Y.; Spain, J.C.
    Biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. strain JS329 (2010), Appl. Environ. Microbiol., 76, 1417-1422.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 1 mM, 98% loss of activity Bradyrhizobium sp.
o-phenanthroline 1 mM, complete loss of activity Bradyrhizobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions Bradyrhizobium sp.

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp. D3WZ86
-
-

Renatured (Commentary)

Renatured (Comment) Organism
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions Bradyrhizobium sp.

Storage Stability

Storage Stability Organism
-20°C, 40% to 50% loss of original activity after 1 week of storage Bradyrhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitro-6-oxohepta-2,4-dienedioate
-
Bradyrhizobium sp. 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite 2nd step of reaction, spontaneous ?
5-nitrosalicylate + O2 oxidation catalyzed by cell extracts requires 0.97 mol O2/mol 5-nitrosalicylate Bradyrhizobium sp. 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite overall reaction ?
5-nitrosalicylate + O2
-
Bradyrhizobium sp. 4-nitro-6-oxohepta-2,4-dienedioate 1st step of reaction ?
salicylate + O2
-
Bradyrhizobium sp. ?
-
?

Synonyms

Synonyms Comment Organism
5NSA dioxygenase
-
Bradyrhizobium sp.
naaB
-
Bradyrhizobium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no requirement of NADPH Bradyrhizobium sp.