Cloned (Comment) | Organism |
---|---|
into the vector pET-24a+ for expression in Escherichia coli ER2566 cells | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
beta-carotene | - |
Gallus gallus | |
0.18 | - |
beta-apo-4'carotenal | - |
Gallus gallus | |
0.19 | - |
beta-apo-8'carotenal | - |
Gallus gallus | |
0.29 | - |
beta-cryptoxanthin | - |
Gallus gallus | |
0.37 | - |
alpha-carotene | - |
Gallus gallus | |
0.69 | - |
gamma-carotene | - |
Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
determined by SDS-PAGE | Gallus gallus |
61210 | - |
theoretical, plus hexahistidine tag | Gallus gallus |
240000 | - |
determined by gel filtration | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | Gallus gallus | two molecules retinal are formed | retinal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | Q9I993 | - |
- |
Purification (Comment) | Organism |
---|---|
by His-Trap HP affinity and Resource Q ion exchange chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
crude extract | Gallus gallus |
0.16 | - |
purification step His-Trap HP | Gallus gallus |
0.32 | - |
purification step Resource Q | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-carotene + O2 | one molecule retinal is formed | Gallus gallus | retinal + alpha-retinal | - |
? | |
beta-apo-4'-carotenal + O2 | one molecule retinal is formed | Gallus gallus | retinal + ? | - |
? | |
beta-apo-8'-carotenal + O2 | one molecule retinal is formed | Gallus gallus | retinal + ? | - |
? | |
beta-carotene + O2 | two molecules retinal are formed | Gallus gallus | retinal | - |
? | |
beta-cryptoxanthin + O2 | - |
Gallus gallus | retinal + (3R)-3-hydroxyretinal | - |
? | |
gamma-carotene + O2 | - |
Gallus gallus | retinal + acycloretinal | one molecule retinal is formed | ? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 60000 | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
beta-carotene 15,15'-monooxygenase | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
gamma-carotene | - |
Gallus gallus | |
0.0083 | - |
alpha-carotene | - |
Gallus gallus | |
0.015 | - |
beta-apo-4'-carotenal | - |
Gallus gallus | |
0.0183 | - |
beta-apo-8'-carotenal | - |
Gallus gallus | |
0.0275 | - |
beta-carotene | - |
Gallus gallus | |
0.0333 | - |
beta-cryptoxanthin | - |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Gallus gallus |