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Literature summary for 1.13.11.63 extracted from

  • Kim, Y.S.; Oh, D.K.
    Substrate specificity of a recombinant chicken beta-carotene 15,15-monooxygenase that converts beta-carotene into retinal (2009), Biotechnol. Lett., 31, 403-408.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-24a+ for expression in Escherichia coli ER2566 cells Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
beta-carotene
-
Gallus gallus
0.18
-
beta-apo-4'carotenal
-
Gallus gallus
0.19
-
beta-apo-8'carotenal
-
Gallus gallus
0.29
-
beta-cryptoxanthin
-
Gallus gallus
0.37
-
alpha-carotene
-
Gallus gallus
0.69
-
gamma-carotene
-
Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
determined by SDS-PAGE Gallus gallus
61210
-
theoretical, plus hexahistidine tag Gallus gallus
240000
-
determined by gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Gallus gallus two molecules retinal are formed retinal
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus Q9I993
-
-

Purification (Commentary)

Purification (Comment) Organism
by His-Trap HP affinity and Resource Q ion exchange chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
crude extract Gallus gallus
0.16
-
purification step His-Trap HP Gallus gallus
0.32
-
purification step Resource Q Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-carotene + O2 one molecule retinal is formed Gallus gallus retinal + alpha-retinal
-
?
beta-apo-4'-carotenal + O2 one molecule retinal is formed Gallus gallus retinal + ?
-
?
beta-apo-8'-carotenal + O2 one molecule retinal is formed Gallus gallus retinal + ?
-
?
beta-carotene + O2 two molecules retinal are formed Gallus gallus retinal
-
?
beta-cryptoxanthin + O2
-
Gallus gallus retinal + (3R)-3-hydroxyretinal
-
?
gamma-carotene + O2
-
Gallus gallus retinal + acycloretinal one molecule retinal is formed ?

Subunits

Subunits Comment Organism
homotetramer 4 * 60000 Gallus gallus

Synonyms

Synonyms Comment Organism
beta-carotene 15,15'-monooxygenase
-
Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0082
-
gamma-carotene
-
Gallus gallus
0.0083
-
alpha-carotene
-
Gallus gallus
0.015
-
beta-apo-4'-carotenal
-
Gallus gallus
0.0183
-
beta-apo-8'-carotenal
-
Gallus gallus
0.0275
-
beta-carotene
-
Gallus gallus
0.0333
-
beta-cryptoxanthin
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Gallus gallus