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Literature summary for 1.13.11.63 extracted from

  • Park, C.; Lee, S.; Kim, Y.; Kim, E.; Sin, H.; Oh, D.; Kim, S.; Um, S.
    Utilization of the recombinant human beta-carotene-15,15-monooxygenase gene in Escherichia coli and mammalian cells (2008), Biotechnol. Lett., 30, 735-741.
    View publication on PubMed

Application

Application Comment Organism
analysis BCMO function in mammalian cells is analyzed by a retinoic acid receptor assay, which responds to the metabolic conversion of beta-carotene to retinoic acid in vivo, this tool can be used to screen more active BCMO for the industrial and pharmacological purpose of retinal production from beta-carotene Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into a Flag fusion-modified mammalian expression vector pcDNA3 and into the bacterial vectors pET-15b and pGEX-4T2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
T381L mutant, shows 1.5fold higher catalytic efficiency for beta-carotene and 1.7fold higher retinal production than the wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
beta-carotene T381L mutant Homo sapiens
0.031
-
beta-carotene wild-type Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
theoretical Homo sapiens
65000
-
determined by SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Homo sapiens
-
retinal
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAY6
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiTrap Chelating HP column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-704 cell human kidney cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
Homo sapiens retinal
-
?

Synonyms

Synonyms Comment Organism
BCMO
-
Homo sapiens
beta-carotene-15,15'-monooxygenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Homo sapiens