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Literature summary for 1.13.11.63 extracted from

  • Fierce, Y.; de Morais Vieira, M.; Piantedosi, R.; Wyss, A.; Blaner, W.S.; Paik, J.
    In vitro and in vivo characterization of retinoid synthesis from beta-carotene (2008), Arch. Biochem. Biophys., 472, 126-138.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.63

Activating Compound

Activating Compound Comment Organism Structure
type I cellular retinol-binding protein i.e. RBP1, RBP1, but not RBP2, is required for the transport of water-insoluble substrates of retinoid pathway enzymes, e.g. of BCMO1, into the cell, and acts as an intracellular sensor of retinoid status that, when present as apo-RBP1, stimulates BCMO1 activity and the conversion of carotenoids to retinoids Mus musculus
type I cellular retinol-binding protein i.e. RBP1, RBP1, but not RBP2, is required for the transport of water-insoluble substrates of retinoid pathway enzymes, e.g. of BCMO1, into the cell, and acts as an intracellular sensor of retinoid status that, when present as apo-RBP1, stimulates BCMO1 activity and the conversion of carotenoids to retinoids Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
EST library screening, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information phenotype of enzyme-deficient mice, beta-carotene conversion is blocked in BCMO1-deficient mice, comparison of plasma retinol and liver retinyl esters of wild-type, heterozygous and homozygous BCMO1-deficient mice, overview Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 x * 63000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Mus musculus BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, and is responsible for metabolically limiting the amount of intact beta-carotene that can be absorbed by mice from their diet, regulation of BCMO1 activity, overview all-trans-retinal
-
 ?
beta-carotene + O2 Homo sapiens BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, regulation of BCMO1 activity, overview all-trans-retinal
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
 Mus musculus all-trans-retinal
-
 ?
beta-carotene + O2
-
 Homo sapiens all-trans-retinal
-
 ?
beta-carotene + O2 BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, and is responsible for metabolically limiting the amount of intact beta-carotene that can be absorbed by mice from their diet, regulation of BCMO1 activity, overview Mus musculus all-trans-retinal
-
 ?
beta-carotene + O2 BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, regulation of BCMO1 activity, overview Homo sapiens all-trans-retinal
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Bcmo1
-
 Mus musculus
Bcmo1
-
 Homo sapiens
beta-carotene-15,15'-monooxygenase
-
 Mus musculus
beta-carotene-15,15'-monooxygenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mus musculus
8
-
 assay at Homo sapiens