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Literature summary for 1.13.11.63 extracted from

  • Poliakov, E.; Gentleman, S.; Cunningham, F.X.; Miller-Ihli, N.J.; Redmond, T.M.
    Key role of conserved histidines in recombinant mouse beta-carotene 15,15-monooxygenase-1 activity (2005), J. Biol. Chem., 280, 29217-29223.
    View publication on PubMed
Search for more literature for 1.13.11.63

Protein Variants

Protein Variants Comment Organism
D52A more than 50% loss of activity Mus musculus
D52A/E140A no enzymatic activity Mus musculus
E140A more than 50% loss of activity Mus musculus
E314A little reduction in enzymatic activity Mus musculus
E405A no enzymatic activity Mus musculus
E450A little reduction in enzymatic activity Mus musculus
E457A about 80% loss of activity Mus musculus
E469A about 50% decrease in activity, cells are bleached when left growing overnight Mus musculus
H172A no enzymatic activity Mus musculus
H174A little reduction in enzymatic activity Mus musculus
H237A no enzymatic activity Mus musculus
H308A no enzymatic activity Mus musculus
H309A little reduction in enzymatic activity Mus musculus
H49A little reduction in enzymatic activity Mus musculus
H514A no enzymatic activity Mus musculus
H58A little reduction in enzymatic activity Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 beta-carotene mutant E140A, pH 8.0, 37°C Mus musculus
0.0012
-
 beta-carotene wild-type, pH 8.0, 37°C Mus musculus
0.0023
-
 beta-carotene mutant E450A, pH 8.0, 37°C Mus musculus
0.0027
-
 beta-carotene mutant H58A, pH 8.0, 37°C Mus musculus
0.0033
-
 beta-carotene mutant E314A, pH 8.0, 37°C Mus musculus
0.0047
-
 beta-carotene mutant D52A, pH 8.0, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron required Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9JJS6 recombinant enzyme
-

Storage Stability

Storage Stability Organism
-20°C, 40% glycerol and presence of protease inhibitors, enzyme purified by metal affinity chromatography is stable for several weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
 Mus musculus all-trans-retinal
-
 ?

Synonyms

Synonyms Comment Organism
Bcmo1
-
 Mus musculus