Cloned (Comment) | Organism |
---|---|
expression of separate domains of NspLOX and the entire protein in Escherichia coli | Nostoc sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70900 | - |
x * 70900, calculated from sequence | Nostoc sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenate + O2 | Nostoc sp. | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
alpha-linolenate + O2 | Nostoc sp. PCC 7120 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
linoleate + O2 | Nostoc sp. | the enzyme is involved in oxylipin biosyntesis | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | Nostoc sp. PCC 7120 | the enzyme is involved in oxylipin biosyntesis | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. | - |
- |
- |
Nostoc sp. PCC 7120 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | Nostoc sp. | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate. Preferred substrate | Nostoc sp. | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | Nostoc sp. PCC 7120 | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate. Preferred substrate | Nostoc sp. PCC 7120 | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
linoleate + O2 | the enzyme is involved in oxylipin biosyntesis | Nostoc sp. | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | i.e. (9Z,12Z)-octadeca-9,12-dienoate, the C-terminal portion of NspLOX is identified as a linoleate linoleate (9R)-lipoxygenase. 42% of the activity with alpha-linolenic acid | Nostoc sp. | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | the enzyme is involved in oxylipin biosyntesis | Nostoc sp. PCC 7120 | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | i.e. (9Z,12Z)-octadeca-9,12-dienoate, the C-terminal portion of NspLOX is identified as a linoleate linoleate (9R)-lipoxygenase. 42% of the activity with alpha-linolenic acid | Nostoc sp. PCC 7120 | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70900, calculated from sequence | Nostoc sp. |
Synonyms | Comment | Organism |
---|---|---|
(9R)-LOX | - |
Nostoc sp. |
NspLOX | NspLOX C-terminal portion exhibits activity as a inoleate 9R-lipoxygenase | Nostoc sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | broad pH-optimum | Nostoc sp. |