Literature summary for 1.13.11.60 extracted from

Su, C.; Brodowsky, I.D.; Oliw, E.H.
Studies on linoleic acid 8R-dioxygenase and hydroperoxide isomerase of the fungus Gaeumannomyces graminis (1995), Lipids, 30, 43-50.

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General Stability

General Stability	Organism
8R-dioxygenase is unstable during purification. It can be stabilized by glutathione, glutathione peroxidase and ethylenediaminetetraacetic acid	Gaeumannomyces graminis

Inhibitors

Inhibitors	Comment	Organism	Structure
4-aminobenzamidine	1 mM, over 70% inhibition	Gaeumannomyces graminis
acetonitrile	10%, inactivation	Gaeumannomyces graminis
Emulgen 911	0.5%, inactivation	Gaeumannomyces graminis
glycine	2%	Gaeumannomyces graminis
ICI 230,487	0.03 mM, 58% inhibition	Gaeumannomyces graminis
Isopropanol	10%, inactivation	Gaeumannomyces graminis
linoleic acid	0.1 mM, partial inactivation, may indicate product inhibition	Gaeumannomyces graminis
additional information	sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme	Gaeumannomyces graminis
N-tosyl-L-phenylalanyl chloromethyl ketone	1 mM, over 70% inhibition	Gaeumannomyces graminis
paracetamol	0.03 mM, 44% inhibition	Gaeumannomyces graminis
polyoxyethylene-10 lauryl ether	0.5%, inactivation	Gaeumannomyces graminis
sodium deoxycholate	0.5%, inactivation	Gaeumannomyces graminis
Triton X-100	0.5%, inactivation	Gaeumannomyces graminis
Tween 20	0.5%, inactivation	Gaeumannomyces graminis
Tween 80	0.5%, inactivation	Gaeumannomyces graminis
Urea	1-4 M	Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
linoleate + O2	Gaeumannomyces graminis	-	(8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Gaeumannomyces graminis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
linoleate + O2	-	Gaeumannomyces graminis	(8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate	-	?

Synonyms

Synonyms	Comment	Organism
linoleic acid 8R-dioxygenase	-	Gaeumannomyces graminis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Gaeumannomyces graminis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Gaeumannomyces graminis	isoelectric focusing	6.3	6.1

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Release 2023.1 (January 2023)